[article]
| Titre : |
Oxygen scavenging polymer coating prepared by hydrophobic modification of glucose oxidase |
| Type de document : |
texte imprimé |
| Auteurs : |
Dana E. Wong, Auteur ; Stephanie M. Andler, Auteur ; Christina Lincoln, Auteur ; Julie M. Goddard, Auteur ; Joey N. Talbert, Auteur |
| Année de publication : |
2017 |
| Article en page(s) : |
p. 489–495 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Aliments -- Emballages -- Aspect sanitaire
Capteurs chimiques
Copolymère éthylène acétate de vinyle
Désoxygénation
Glucose oxydaseLa glucose oxydase (GOx, GOD) est une enzyme oxydo-réductase (EC 1.1.3.4) qui catalyse l'oxydation du glucose en peroxyde d'hydrogène et en D-glucono-δ-lactone. Dans les cellules, elle participe à cliver les sucres (oses), notamment le saccharose (Glc-Fru) en métabolites.
La GOx est largement utilisée pour déterminer la concentration en glucose libre dans les fluides corporels (diagnostic), et dans les aliments (industrie). Elle a de nombreuses applications en biotechnologies, typiquement les tests enzymatiques de biochimie.
Ions
Revêtements
|
| Index. décimale : |
667.9 Revêtements et enduits |
| Résumé : |
Trace oxygen in packaged foods, beverages, and pharmaceuticals can promote a range of oxidative degradation reactions and support microbial growth, ultimately impacting product quality and shelf-life. Oxygen scavenging active packaging systems have therefore been explored to control headspace oxygen content. Herein, we report on a hydrophobic ion pairing method to render glucose oxidase hydrophobic, and thus soluble in organic solvents. Hydrophobic modified glucose oxidase was blended with ethylene vinyl acetate and cast on the interior of glass vials to demonstrate potential as a commercially translatable coating method for enzyme immobilization. The resulting oxygen scavenging polymer coatings were topographically uniform and presented 0.553 μg/cm2 enzyme at the coating interface. The coatings effectively reduced headspace oxygen by 2% in a closed-vial system filled 50 vol% with citrate buffer, pH 3.5. Less than 25% protein migrated from the coating over an 8-week leaching study, with no detectable protein leached in the first 4 weeks. Hydrophobic ion pairing of glucose oxidase enabled a facile, high-throughput enzyme immobilization technique without use of complicated, time-consuming surface modification chemistries and reagents. Such oxygen scavenging polymer coatings can support controlling headspace oxygen in packaged goods, and thus retaining stability of oxygen-sensitive components such as colors, flavors, and nutrients. |
| Note de contenu : |
- Characterization of glucose oxidase embedded polymer coatings
- Protein migration
- Oxygen scavenging capacity of glucose oxidase embedded polymer coatings |
| DOI : |
10.1007/s11998-016-9865-6 |
| En ligne : |
https://link.springer.com/content/pdf/10.1007%2Fs11998-016-9865-6.pdf |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=28163 |
in JOURNAL OF COATINGS TECHNOLOGY AND RESEARCH > Vol. 14, N° 2 (03/2017) . - p. 489–495
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Oxygen scavenging polymer coating prepared by hydrophobic modification of glucose oxidase in JOURNAL OF COATINGS TECHNOLOGY AND RESEARCH, Vol. 14, N° 2 (03/2017)
