Titre : |
Engineering an improved cellulase for fabric care in liquid detergents |
Type de document : |
texte imprimé |
Année de publication : |
2016 |
Article en page(s) : |
p. 64-67 |
Langues : |
Anglais (eng) |
Catégories : |
Cellulases Une cellulase est une enzyme qui peut décomposer la cellulose. Les cellulases sont classées EC 3.2.1.4. Elles sont produites typiquement par des bactéries, levures et de protozoaires, qui jouent un rôle majeur dans la digestion des animaux, et transformation de la matière organique végétale en humus dans le sol. Elles ont aussi des applications biotechnologiques et industrielles. Détergents Essais (technologie) Textiles et tissus -- Lavage
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Index. décimale : |
668.1 Agents tensioactifs : savons, détergents |
Résumé : |
The counter-effect of protease on cellulase performance in liquid detergents is well-known. DuPont set out to develop a more stable cellulase with a much higher resistance to the degrading effects of subtilisin than currently available enzymes. Using molecular modelling and structure function analysis, it was determined which regions of the cellulase might be susceptible to protease degradation. Molecular genetic techniques were used to generate variant enzymes in the regions identified. High Throughput Screening technology helped to determine a selection of enzymes for further testing/optimization.
At a later stage of development, biochemical and application testing was employed to test the more promising variants for two parameters: (1) Performance in a washing machine in the presence of a typical protease, to test for protease-resistance in consumer-relevant conditions. (2) Stability in a liquid detergent in storage at elevated temperature over a period of 28 days. The results of the development and testing of this new cellulase are detailed in this article. The main conclusions were :
- In a comparison against a competitive cellulase, using different washing conditions for different regions, de-pilling and color renewal were substantially improved
- A significant improvement of the cellulase activity in a protease-containing detergent over 28 days of storage was established. |
Note de contenu : |
- RESEARCHING THE DEMAND FOR A MORE EFFECTIVE CELLULASE
- KEY FINDINGS
- THE TECHNICAL CHALLENGES OF THE CONSUMER-DRIVEN NEED
- ENGINEERING A NEW ENZYME WITH OPTIMUM STABILITY AND PERFORMANCE
- BIOCHEMICAL AND APPLICATION TESTING METHODS AND PROTOCOLS : Anti-pilling and color maintenance performance in a washing machine - De-pilling and pilling prevention performance of the improved cellulase tested during the wash cycle in consumer-relevant conditions - Color protection performance of the improved cellulase tested durig the wash cycle in consumer-relevant conditions
- CONCLUSIONS OF IN-WASH TESTING : Storage stabiility in a liquid detergent over a period of 28 days - Storage stability of different cellulases over a period of 28 days |
En ligne : |
https://drive.google.com/file/d/1urlMnMAuLdKDzPwIbrPmaWZgxDCAVXWt/view?usp=drive [...] |
Format de la ressource électronique : |
Pdf |
Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=27873 |
in SOFW JOURNAL > Vol. 142, N° 12 (12/2016) . - p. 64-67