| Titre : |
Identification of fur species by DNA barcoding based on 16S rRNA gene |
| Type de document : |
texte imprimé |
| Auteurs : |
Fangfang Qin, Auteur ; Sun Shiyu, Auteur ; Zongliang Chen, Auteur ; Yuluan Wu, Auteur ; Delian Wang, Auteur ; Yanping Xian, Auteur ; Hao Dong, Auteur ; Bin Wang, Auteur ; Li Wang, Auteur |
| Année de publication : |
2016 |
| Article en page(s) : |
p. 298-303 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
ADN
Extraction (chimie)
Fourrures -- Identification
Marqueurs génétiques
Réaction en chaîne par polymérase
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Traditional identification of fur species is a problem ; without unified standards il depends significantly on the experience of the tester. In this study, we cleveloped a.method of identification of fur species by DNA barcoding based on 16S rRNA gene. 18 usuel fur species sequences of 165 rRNA gene were obtained from Genebank and primers for PCR were designed in the conserved ragions. By the optimization of experimental procedure, polymerase chain reaction (PC R) can be successfully performed with DNA isolated from fur. Sequence analyses of the -240bp production of PCR could help us to authenticate the fur species accurately. |
| Note de contenu : |
- Reference materials and samples
- DNA extraction
- Primer designing
- PCR and DNA sequencing |
| En ligne : |
https://drive.google.com/file/d/1oHUj5KyzEZUcgu-BSqYngLtKVARJqchd/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=27665 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 100, N° 6 (11-12/2016) . - p. 298-303
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Identification of fur species by DNA barcoding based on 16S rRNA gene in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 100, N° 6 (11-12/2016)
