Cross-linking reaction between genipin and porcine acellular dermal matrix / Dan Nianhua in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 99, N° 4 (07-08/2015)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 99, N° 4 (07-08/2015) . - p. 176-182 Titre : Cross-linking reaction between genipin and porcine acellular dermal matrix Type de document : texte imprimé Auteurs : Dan Nianhua, Auteur ; Xiao Shiwei, Auteur ; Hu Yang, Auteur ; Chen Yining, Auteur ; Dan Weihua, Auteur Année de publication : 2015 Article en page(s) : p. 176-182 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Cuirs et peaux de porcs Génipine Réticulation (polymérisation) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Genipin(GP) has been used as a sort of perfect naturel cross-linking agent for collagenous biomaterials, but the cross-linking mechanism between collagen and GP is not clear. The aldehyde groups can be formed in aqueous solution of Genipin (GP), which can react with Cu(OH)2 and be used in the silver mirror reaction. About 0.2506 moi aldehyde groups were determined by Decolor Fuchsin Spectrophotometry in 1mol GP. In neutral solution, the ultraviolet absorption peak of GP appeared at 242.10nm, whereas in alkaline solution, it GP moved to 271.70nm due to the generation of enolates structure. Compared with the neutral condition, the GP molecular size almost doubled in alkaline solution, therefore il was speculated that dimers were formed, In this work, porcine acellular dermal matrix (pADM) was employed as a model of collagen to react with Genipin. The colour of GP cross-linked pADM (GP-pADM) turned into yellow from white initialty and finally into dark blue and a new absorption peak was round at 2356.79cm-1. With an increasing amount of GP, the shrinkage temperature (Ts) of GP-pADM increased slowly from 55.4°C to 80°C (6% dosage). Regarding the formation of dimers, the size of GP molecule was increased which fitted the distance of two aminos of the collagen for cross-linking. Furthermore, due to the ring structure of genipin and cross-linking bonds, the amount of rigid structures of collagen increased, consequently improved collagen stability was achieved. Fibroblasts grew well on GP-pADM with great morphology, and the vitro cytotoxicity was grade 0. Note de contenu : - EXPERIMENTAL PROCEDURES : Materials and equipment - Determination of aldehyde groups - Particle size of GP in solution at various ph and temperature - Ultraviolet absorption of GP solution - 13C NMR - Shrinkage temperature (Ts) and tensile strength - Infrared spectrum (IR) detection - Cytotoxicity - RESULTS AND DISCUSSION : Amount of aldehyde - Ultraviolet spectrum of GP solution - Particle size of GP at various pHs and temperatures - 13C NMR - IR of GP-PADM - Shrinkage temperature and tensil strength of GP-pADM - Hypothesis of the cross-linking mechanism between collagen and GP - Cytoxicity of GP-pADM En ligne : https://drive.google.com/file/d/1AvZ07QZCIrmr94nZ30lW75ObqXV3NPMh/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=24559 [article]

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Potential applications of solid waste generated in the leather industry as a novel supporting matrix for immobilization of enzyme / Hu Yang in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 98, N° 3 (05-06/2014)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 98, N° 3 (05-06/2014) . - p. 121-126 Titre : Potential applications of solid waste generated in the leather industry as a novel supporting matrix for immobilization of enzyme Type de document : texte imprimé Auteurs : Hu Yang, Auteur ; Lan Liu, Auteur ; Dan Weihua, Auteur ; Dan Nianhua, Auteur Année de publication : 2014 Article en page(s) : p. 121-126 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Cuirs et peaux -- Déchets Cuirs et peaux de bovins Epilage Essais (technologie) Peptidases Stabilité thermique Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : A novel method for the immobilization of an enzyme was established by using solid waste from tanneries as the carrier. A series of inorganic and organic carriers with good behaviours were evaluated in this context. The results showed that the enzyme immobilization efficiency was optimal when the enzyme offer, reaction temperature, pH, ionic strength and time were set at 335mg enzyme per g carrier, 25°C, 6.5, 0.1mol/L and 60 minutes, respectively. The optimal temperature and pH of the immobilized enzyme in terms of protease were 55°C and 8.0, respectively, similar to that for the free enzyme. The immobilized enzyme remained 64% of its original activity after keeping at 55°C for 3 hours, while the free enzyme lost nearly 54% under the same conditions, indicating the improvement of enzyme thermal stability after enzyme immobilization. Furthermore, the leather industry oriented application of the immobilized enzyme was intended for the dehairing of cattle hide. Note de contenu : - EXPERIMENTAL PROCEDURES : Materials - Methods - RESULTS AND DISCUSSION : Immobilization of enzyme onto different carriers - Effect of immobilization conditions on enzyme preparations - Optimal conditions for the immobilized enzyme - Thermal stability of immobilized enzyme - Dehairing test En ligne : https://drive.google.com/file/d/1WpU4zfT69-xVuaJH8ZEOpbX_qqhM10g4/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=21531 [article]

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Protective effect of calcium hydroxide in hair-saving unhairing process / Hu Yang in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 97, N° 5 (09-10/2013)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 97, N° 5 (09-10/2013) . - p. 200-206 Titre : Protective effect of calcium hydroxide in hair-saving unhairing process Type de document : texte imprimé Auteurs : Hu Yang, Auteur ; Lan Liu, Auteur ; Dan Weihua, Auteur ; Dan Nianhua, Auteur Année de publication : 2013 Article en page(s) : p. 200-206 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Chaux Cuirs et peaux -- Défauts Cystine La cystine est un acide aminé composé de deux unités cystéine liées par un pont disulfure. Epilage Immunisation (épilage) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : To better understand the mechanism of alkali-induced hair immunization, in this work native bovine hair was selected as the direct research subject rather than fresh salted bovine hide. The state of hair was evaluated, both before and after having undergone the procedure of immunization by measuring the changes of active groups, thermal stability, surface morphology, strength, calcium content and cystine content. The result indicates that the immunization is indeed caused by the interaction between calcium hydroxide and the cystine active center. Meanwhile, the optimum condition for immunization was: calcium hydroxide 15g/I and treatment time 90 minutes. This work may, to some extent, provide theoretical support for searching for the balance between the dehairing [hair burning] and hair saving process. Note de contenu : - IMMUNIZATION PHENOMENON - EXPERIMENTAL PROCEDURE : Raw materials and chemicals - Tests for hair characterization - Analysis of waste liquor - ASSAYS : Protective effect of immunization against the attack of reducing agent - Hair characterization - Pollution evaluation of the hair-saving unhairing En ligne : https://drive.google.com/file/d/1q7gZWIMmjuSJ327BMFmwfx82EZWa1BXX/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=19568 [article]

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