| Titre : |
Recovery and characterization of protein hydrolysate from chrome shavings by microbial degradation |
| Type de document : |
texte imprimé |
| Auteurs : |
C. Shanthi, Auteur ; Pradipta Banerjee, Auteur ; Chandra Babu Narasimhan Kannan, Auteur ; G. Suseela Rajkumar, Auteur |
| Année de publication : |
2013 |
| Article en page(s) : |
p. 231-239 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Bactéries
Biodégradation
Caractérisation
Collagène
Cuirs et peaux -- Déchets -- Recyclage
Hydrolysats de protéines
Récupération (Déchets, etc.)
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Chromium containing collagenous solid wastes from the leather tanning industry requires special attention because of the pressure exerted by environmental authorities for safe disposal. Such collagen-rich wastes can be recycled by adapting environmentally suitable and safer methods based on microorganism and/or enzymatic treatment, so as to obtain a collagen hydrolysate with potential applications. In the present study, chromium tolerant bacteria were isolated from tannery soil and screened for high proteolytic activity by zone-clearance assay and caseinolytic activity. The most potent bacterium, with a high proteolytic and chromium-tolerant ability was found to degrade about 90% of the chrome shavings in 120h and was identified as Alcaligenes faecalis. The hydrolysates at various time intervals of proteolysis were collected, chromium removed and characterized. The hydrolysate was found to have 12% ash and 80% protein or peptides contents after 120h of proteolysis with 3.14±2.0?g of chromium/g of protein. Molecular weight profiling done by gel filtration chromatography using sephadex G 25 and tricine-SDS-PAGE electrophoresis revealed that the major component of the hydrolysate comprised of small peptides in the molecular weight range of 3-30kDa. |
| Note de contenu : |
- MATERIALS AND METHODS : Isolation of organism - Protease activity - Chromium tolerance - Degre of hydrolysis (%) and Cr3 removal from the chrome shavings - Analysis of the protein hydrolysate - pH determination by deionization - Gel filtration chromatography - Ion exchange chromatography - Tricine SDS-PAGE of the hydrolysate
- RESULTS AND DISCUSSION : Protease activity, identification and chromium tolerance of the screened bacterium - Chrome shaving hydrolysis process - Characterization of protein hydrolysate - Determination of molecular weight of protein hydrolysate by gel filtration and gel electrophoresis |
| En ligne : |
https://drive.google.com/file/d/1LQ3kblknV4gm5BdYm3xIrye-aspbynzD/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=18746 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CVIII, N° 6 (06/2013) . - p. 231-239
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