[article]
| Titre : |
4,5-Dinitrosulfonefluorescein and related dyes : Kinetics of reversible rupture of the pyran ring and their interaction with lysozyme |
| Type de document : |
texte imprimé |
| Auteurs : |
Nika N. Kriklya, Auteur ; Taras Yu. Gromovoy, Auteur ; Nicolay O. Mchedlov-Petrossyan, Auteur |
| Année de publication : |
2021 |
| Article en page(s) : |
p. 658-667 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Caractérisation
Colorants -- Analyse
Dynamique moléculaire
Fluorescence
LysozymeLe lysozyme est une protéine globulaire formée de 129 acides aminés (chez l'être humain), que l'on rencontre dans un certain nombre de sécrétions (larmes, salive, lait maternel, mucus...) et dans le blanc d'œuf, chez de nombreuses espèces d'animaux, dont insectes et acariens1.
Il s'agit d'une hydrolase acide (EC 3.2.1.17) sécrétée par les granulocytes et les monocytes. Elle détruit la paroi bactérienne en catalysant l'hydrolyse des peptidoglycanes la constituant.
Spectrométrie de masse
|
| Index. décimale : |
667.3 Teinture et impression des tissus |
| Résumé : |
Fluorescein and sulfonefluorescein dyes are stable in solution within a wide pH range. Nucleophilic addition of the hydroxide (HO–) group to the nodal carbon atom is not typical of these compounds. They are also not prone to rupture of the pyran cycle under the action of alkali. However, the last-named heterocycle readily opens at high pH values in the case of derivatives containing nitro groups in the xanthene moiety. Moreover, while in the case of nitrofluoresceins, for example, 4,5-dinitrofluorescein, this reaction is irreversible, with 4,5-dinitrosulfonefluorescein acidification of the alkaline solution immediately returns the visible absorption spectrum to that of the original hydroxyxanthene dye. We present a quantitative study of the kinetics of the pyran cycle rupture in aqueous alkali without and with micelles of a cationic surfactant. Also, both initial dye and reaction product were studied via the matrix-assisted laser desorption/ionisation time-of-flight (MALDI-ToF) technique. The second part of the article is devoted to the interaction of 4,5-dinitrosulfonefluorescein and other dyes of similar structure with lysozyme basing on the MALDI-ToF mass spectrometry. This made it possible to arrange the dyes according to their affinity for the protein and draw some conclusions about the nature of the association of the dye with lysozyme. |
| Note de contenu : |
- EXPERIMENTAL : Materials - Mass spectrometry - Characterisation of the colloidal species - Procedure of the kinetics study
- RESULTS AND DISCUSSION : Alkaline rupture of the pyrancycle of 4,5-dinitrosulfonefluorescein - Kinetic data processing : Aqueous NaOH solutions - Surfactant effect : 4,5-dinitrosulfonefluorescein in the aqueous CTAB/NaOH system - The behaviour of 2,4,5,7-tetranitrosulfonefluorescein in alkaline aqueous media - Interaction of the dyes with lysozyme |
| DOI : |
https://doi.org/10.1111/cote.12565 |
| En ligne : |
https://onlinelibrary.wiley.com/doi/epdf/10.1111/cote.12565 |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=36795 |
in COLORATION TECHNOLOGY > Vol. 137, N° 6 (12/2021) . - p. 658-667
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