[article]
| Titre : |
Cross-linking reaction between genipin and porcine acellular dermal matrix |
| Type de document : |
texte imprimé |
| Auteurs : |
Dan Nianhua, Auteur ; Xiao Shiwei, Auteur ; Hu Yang, Auteur ; Chen Yining, Auteur ; Dan Weihua, Auteur |
| Année de publication : |
2015 |
| Article en page(s) : |
p. 176-182 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Cuirs et peaux de porcs
Génipine
Réticulation (polymérisation)
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Genipin(GP) has been used as a sort of perfect naturel cross-linking agent for collagenous biomaterials, but the cross-linking mechanism between collagen and GP is not clear. The aldehyde groups can be formed in aqueous solution of Genipin (GP), which can react with Cu(OH)2 and be used in the silver mirror reaction. About 0.2506 moi aldehyde groups were determined by Decolor Fuchsin Spectrophotometry in 1mol GP. In neutral solution, the ultraviolet absorption peak of GP appeared at 242.10nm, whereas in alkaline solution, it GP moved to 271.70nm due to the generation of enolates structure. Compared with the neutral condition, the GP molecular size almost doubled in alkaline solution, therefore il was speculated that dimers were formed, In this work, porcine acellular dermal matrix (pADM) was employed as a model of collagen to react with Genipin. The colour of GP cross-linked pADM (GP-pADM) turned into yellow from white initialty and finally into dark blue and a new absorption peak was round at 2356.79cm-1. With an increasing amount of GP, the shrinkage temperature (Ts) of GP-pADM increased slowly from 55.4°C to 80°C (6% dosage). Regarding the formation of dimers, the size of GP molecule was increased which fitted the distance of two aminos of the collagen for cross-linking. Furthermore, due to the ring structure of genipin and cross-linking bonds, the amount of rigid structures of collagen increased, consequently improved collagen stability was achieved. Fibroblasts grew well on GP-pADM with great morphology, and the vitro cytotoxicity was grade 0. |
| Note de contenu : |
- EXPERIMENTAL PROCEDURES : Materials and equipment - Determination of aldehyde groups - Particle size of GP in solution at various ph and temperature - Ultraviolet absorption of GP solution - 13C NMR - Shrinkage temperature (Ts) and tensile strength - Infrared spectrum (IR) detection - Cytotoxicity
- RESULTS AND DISCUSSION : Amount of aldehyde - Ultraviolet spectrum of GP solution - Particle size of GP at various pHs and temperatures - 13C NMR - IR of GP-PADM - Shrinkage temperature and tensil strength of GP-pADM - Hypothesis of the cross-linking mechanism between collagen and GP - Cytoxicity of GP-pADM |
| En ligne : |
https://drive.google.com/file/d/1AvZ07QZCIrmr94nZ30lW75ObqXV3NPMh/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=24559 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 99, N° 4 (07-08/2015) . - p. 176-182
[article]
|
Aller sur edunet
Accueil
Cross-linking reaction between genipin and porcine acellular dermal matrix in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 99, N° 4 (07-08/2015)
