| Titre : |
A molecular level investigation of dialdehyde starch interaction with collagen for eco-friendly stabilization |
| Type de document : |
texte imprimé |
| Auteurs : |
Gladstone Christopher Jayakumar, Auteur ; Swarna Vinodh Kanth, Auteur ; Jonnalagadda Raghava Rao, Auteur ; Balachandran Unni Nair, Auteur |
| Année de publication : |
2015 |
| Article en page(s) : |
p. 145-151 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Amidon dialdéhyde
Antienzymes
Collagène
Dynamique moléculaire
Réticulation (polymérisation)
Stabilisants (chimie)
Stabilité thermique
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
The current study investigates Dialdehyde Starch (DAS) as a stabilizing agent for collagen. DAS is a well established crosslinking agent for protein; however, molecular interaction with collagen was not elucidated. Dialdehyde involves in the formation of inter and intra crosslinking with protein which renders higher stability against heat and enzyme. Crosslinking efficiency of DAS with collagen was found to increase with increase in the concentration. DAS interacted collagen membrane exhibited an increase in the thermal stability of about 35°C at pH 8. Swelling degree of collage-DAS membranes were found to decrease with increase in the concentration of DAS owing to the shift in the nucleation behavior in collagen fibrillogenesis. DAS treated collagen membrane shows 90% resistance to collagenase due to the unavailability of cleaving sites in collagen-DAS fibres. Reconstittued collage-DAS collagen membranes showed increase in cell proliferation which signifies its non-toxic characteristics. Therefore, DAS can be a new class of green tanning agent for skin stabilization and also finds applications in scaffold preparation. |
| Note de contenu : |
- MATERIALS AND METHODS : Reagents and chemicals - Preparation of type I collagen - Preparation of reconstituted collagen membranes - Crosslinking conditions - Crosslinking efficiency - Swelling ratio - Thermal resistance of DAS stabilized collagen membrane hydrothermal temperature - Calorimetric measurement - Denaturation temperature - Collagenolytic activity of DAS stabilized collagen matrix - Scanning Electron Microscopy studies of DAS stabilized collagen membrane - In vitro cytotoxicity analysis
- RESULTS AND DISCUSSION : Effiency of DAS interaction with biomembrane - TNBS assay - Water absorption in collagen-DAS biomembrane - Thermal stability of collagen-Das biomembrane - DAS-collagen biomembrane resistance against collagenase - Morphological study of collagen-DAS biomembrane - Evaluation of cell proliferation on collagen - DAS biomembrane substrate - Plausible mechanism of dialdehyde starch interaction with collagen |
| En ligne : |
https://drive.google.com/file/d/1Xdg1FiqhE-W-sP_5lxd2otPh_8Tl6HsR/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=24055 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CX, N° 5 (05/2015) . - p. 145-151
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A molecular level investigation of dialdehyde starch interaction with collagen for eco-friendly stabilization in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CX, N° 5 (05/2015)
