Enhancement of biosurfactant production from pseudomonas cepacia CCT6659 through optimisation of nutritional parameters using response surface methodology / Rita de Cassia in TENSIDE, SURFACTANTS, DETERGENTS, Vol. 50, N° 2 (03-04/2013)

[article]  inTENSIDE, SURFACTANTS, DETERGENTS > Vol. 50, N° 2 (03-04/2013) . - p. 137-142 Titre : Enhancement of biosurfactant production from pseudomonas cepacia CCT6659 through optimisation of nutritional parameters using response surface methodology Type de document : texte imprimé Auteurs : Rita de Cassia, Auteur ; F. S. da Silva, Auteur ; Raquel D. Rufino, Auteur ; Juliana M. Luna, Auteur ; Charles B. B. Farias, Auteur ; Hilàrio J. B. Filho, Auteur ; Valdemir A. dos Santos, Auteur ; A. Sarubbo, Auteur Année de publication : 2013 Article en page(s) : p. 137-142 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bactéries à gram négatif Biosurfactants Chimie des surfaces Index. décimale : 668.1 Agents tensioactifs : savons, détergents Résumé : The aim of the present study was to optimise the production of a biosurfactant by a new strain of Pseudomonas cepacia CCT6659 with aid of a combination of central composite rotatable design (CCRD) and response surface methodology (RSM). The factors selected for optimisation of the growth conditions were canola waste frying oil, corn steep liquor and NaNO3 substrate concentrations. Surface tension was chosen as the response variable. All factors studied were important within the ranges investigated. The empirical forecast model developed through RSM regarding effective nutritional factors was adequate for explaining 89% of the variation observed in biosurfactant production. Maximal reduction in surface tension of 26 mN m–1 was obtained under the optimal conditions of 2% waste frying oil, 3% corn steep liquor and 0.2% NaNO3. The accumulation of isolated biosurfactant increased from 2 g L–1 to 8.0 g L–1 under these conditions, demonstrating that the factorial design is adequate for identifying the optimal conditions for biosurfactant production. Note de contenu : - Materials - Bacterial strain and preparation of seed culture - Fermentation media - Optimisation of culture conditions by RSM - Determination of surface tension - Biosurfactant isolation DOI : 10.3139/113.110241 Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=17894 [article]

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