| Titre : |
Potential applications of solid waste generated in the leather industry as a novel supporting matrix for immobilization of enzyme |
| Type de document : |
texte imprimé |
| Auteurs : |
Hu Yang, Auteur ; Lan Liu, Auteur ; Dan Weihua, Auteur ; Dan Nianhua, Auteur |
| Année de publication : |
2014 |
| Article en page(s) : |
p. 121-126 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Cuirs et peaux -- Déchets
Cuirs et peaux de bovins
Epilage
Essais (technologie)
Peptidases
Stabilité thermique
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
A novel method for the immobilization of an enzyme was established by using solid waste from tanneries as the carrier. A series of inorganic and organic carriers with good behaviours were evaluated in this context. The results showed that the enzyme immobilization efficiency was optimal when the enzyme offer, reaction temperature, pH, ionic strength and time were set at 335mg enzyme per g carrier, 25°C, 6.5, 0.1mol/L and 60 minutes, respectively. The optimal temperature and pH of the immobilized enzyme in terms of protease were 55°C and 8.0, respectively, similar to that for the free enzyme. The immobilized enzyme remained 64% of its original activity after keeping at 55°C for 3 hours, while the free enzyme lost nearly 54% under the same conditions, indicating the improvement of enzyme thermal stability after enzyme immobilization. Furthermore, the leather industry oriented application of the immobilized enzyme was intended for the dehairing of cattle hide. |
| Note de contenu : |
- EXPERIMENTAL PROCEDURES : Materials - Methods
- RESULTS AND DISCUSSION : Immobilization of enzyme onto different carriers - Effect of immobilization conditions on enzyme preparations - Optimal conditions for the immobilized enzyme - Thermal stability of immobilized enzyme - Dehairing test |
| En ligne : |
https://drive.google.com/file/d/1WpU4zfT69-xVuaJH8ZEOpbX_qqhM10g4/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=21531 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 98, N° 3 (05-06/2014) . - p. 121-126
Aller sur edunet
Accueil
Potential applications of solid waste generated in the leather industry as a novel supporting matrix for immobilization of enzyme in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 98, N° 3 (05-06/2014)
