| Titre : |
Inhibition of propionibacterium acnes lipase by extracts of indian medicinal plants |
| Type de document : |
texte imprimé |
| Auteurs : |
V. Patil, Auteur ; A. Bandivadekar, Auteur ; D. Debjani, Auteur |
| Année de publication : |
2012 |
| Article en page(s) : |
p. 234-239 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Acné L'acné (nom féminin) est une dermatose (maladie de la peau) inflammatoire des follicules pilosébacés (glandes sécrétant le sébum, à la racine des poils) avec formation de comédons.
Cette maladie de la peau est représentée dans sa forme la plus fréquente par l'acné polymorphe juvénile qui touche les adolescents, mais qui ne résume pas toutes les acnés. Il existe aussi des acnés exogènes d'origine professionnelle, médicamenteuse, cosmétique...
Bactéries propioniques
Concentration minimale bactéricide
Extraits de plantes:Extraits (pharmacie)
Peau -- Inflammation
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
Lipases play an important role in pathogenesis of acne by hydrolysing sebum triglycerides and releasing irritating free fatty acids in the pilosebaceous follicles. Lipase is a strong chemotactic and proinflammatory antigen. Therefore, lipase has generated a high interest as a pharmacological target for antiacne drugs. The aim of this study was to identify inhibitory effects of plant extracts on the lipase activity of Propionibacterium acnes. Colorimetric microassay was used to determine lipase activity. Extracts from Terminalia chebula and Embelia ribes showed lower IC50 value (1 μg mL−1) for lipase inhibition as compared to Vitex negundo and Picrorhiza kurroa (19 and 47 μg mL−1, respectively). The active component responsible for lipase inhibition was isolated. This study reports for the first time the novel antilipase activity of chebulagic acid (IC50: 60 μmol L−1) with minimum inhibitory concentration value of 12.5 μg mL−1 against P. acnes. The inhibitory potential of plant extracts was further confirmed by plate assay. The organism was grown in the presence of subinhibitory concentrations of extracts from P. kurroa, V. negundo, T. chebula, E. ribes and antibiotics such as clindamycin and tetracycline. Extract from T. chebula showed significant inhibition of lipase activity and number of P. acnes. |
| Note de contenu : |
- MATERIALS AND METHODS : Plant material - Reagents and chemicals - Evaluation of antimicrobial activity by cup plate diffusion method - Determination of minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) values - Evaluation of lipase activity by colorimetric microassay - Determination of antilipase activity of plant extracts by plate assay method - Determinationof viable count by spread plate method - Extraction of chebulagic acid by preparative high performance thin layer chromatography (HPTLC)
- RESULTS : Antipropionibacterium assay by agar cup diffusion, MIC and MBC - Isolation of chebulagic acid - Inhibition of lipase activity |
| DOI : |
10.1111/j.1468-2494.2012.00706.x |
| En ligne : |
http://onlinelibrary.wiley.com/doi/10.1111/j.1468-2494.2012.00706.x |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=15033 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 34, N° 3 (06/2012) . - p. 234-239
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