Titre : |
Minimal bactericidal concentration for a quaternary ammonium compound used in soak liquors |
Type de document : |
texte imprimé |
Auteurs : |
Canan Veyselova, Auteur ; Meral Birbir, Auteur ; Didem Berber, Auteur |
Année de publication : |
2013 |
Article en page(s) : |
p. 166-171 |
Note générale : |
Bibliogr. |
Langues : |
Anglais (eng) |
Catégories : |
Ammoniums quaternaires Antibactériens Concentration minimale bactéricide Peaux brutes -- Trempe
|
Index. décimale : |
675 Technologie du cuir et de la fourrure |
Résumé : |
The soaking process is an ideal environment for bacterial growth and bacterial activity during this process causes severe damages on hides. The use of an effective bactericidal agent during soaking is very important to prevent the detrimental effect of bacteria on hides. A quaternary ammonium compound (QAC) containing 12.5% didecyl dimethyl ammonium chloride and 12.5% benzyl dimethyl ammonium chloride which is used in soaking liquors in Turkiye was examined for its effect on Bacillus
licheniformis, Bacillus pumilus, Staphylococcus intermedius, Pseudomonas luteola, Enterobacter cloacae, Vibrio fluvialis and Enterococcus faecium.
Staphylococcus intermedius, Pseudomonas luteola, Enterobacter cloacae and Enterococcus faecium were isolated from soak liquor containing 0.8g/L of the test agent. Bacillus licheniformis, Bacillus pumilus and Vibrio fluvialis were isolated from salt-pack-cured hides. These bacteria were commonly found in isolates from salted hides.
Thé effect of the test agent at different concentrations on the bacteria was examined by the Kirby-Bauer disk diffusion method on Mueller Hinton Agar both at 25°C and at 37°C. In addition, the Minimal Bactericidal Concentration of the agent for the bacteria and the mixed population of these bacteria was also determined in Mueller Hinton Broth at 37°C.
All concentrations of the agent produced an inhibition zone for all of the test bacteria. Inhibition zone diameter changed according to concentration of agent and the species of bacteria. The agent was found to be more effective at 25°C than at 37°C. 2.97g/L of the agent in Mueller Hinton Broth was found to kill both the bacteria and mixed culture at exposure times of both 8 and 24 hours at 37°C. It was concluded that the agent should be used in higher concentrations in soak liquors to inactivate the bacteria that might cause major damages on hides. |
Note de contenu : |
- INTRODUCTION : The range of products employed - Resistance to antibacterials - Categories of agent - Previous study
- EXPERIMENTAL PROCEDURES : Test bacteria and agent - Determination of protease activity - Determination of lipase activity - Assessment of the efficiency of the test agent on the test bacteria at different concentrations at 25°C and at 37°C by Kirby-Bauer disc diffusion method - Determination of the Minimal Bactericidal Concentration of the test agent on the test bacteria and their mixed culture at 37°C - Bacteria isolated from soak liquor treated with QAC - Bacteria previously found on salt-pack-cured hides - Proteases and lipases found in this study - Previous studies - Results of present study |
En ligne : |
https://drive.google.com/file/d/12n4-3uVqGRrYZLcklkvihC_-s-X855_H/view?usp=drive [...] |
Format de la ressource électronique : |
Pdf |
Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=19221 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 97, N° 4 (07-08/2013) . - p. 166-171