Methods of isolation and identification of pathogenic and potential pathogenic bacteria from skins and tannery effluents / Anne Lama in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CVIII, N° 2 (02/2013)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CVIII, N° 2 (02/2013) Titre : Methods of isolation and identification of pathogenic and potential pathogenic bacteria from skins and tannery effluents Type de document : texte imprimé Auteurs : Anne Lama, Auteur ; Margaret Bates, Auteur ; Anthony D. Covington, Auteur ; Stuart C. Allen, Auteur ; A. Paula M. Antunes, Auteur Année de publication : 2013 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification Bactéries -- Isolement Bactéries pathogènes Cuirs et peaux -- Analyse Eaux usées -- Analyse Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Currently there is no standard protocol available within the leather industry to isolate and identify pathogenic bacteria from hides, skins or tannery effluent. This study was therefore carried out to identify simple but effective methods for isolation and identification of bacterial pathogens from the effluent and skins during leather processing. Identification methods based on both phenotypic and genotypic characteristics were investigated. Bacillus cereus and Pseudomonas aeruginosa were used as indicator bacteria to evaluate the isolation and identification methods. Decontaminated calfskins were inoculated with a pure culture of the above mentioned bacterial species followed by pre-tanning and chromium tanning processes. Effluent samples were collected and skins were swabbed at the end of each processing stage. Bacterial identification was carried out based on the phenotypic characteristics; such as colony appearance on selective solid media, cell morphology following a standard Gram-staining and spore staining techniques, and biochemical reactions, e.g., the ability of a bacterial species to ferment particular sugars and ability to produce certain enzymes. Additionally, an identification system based on bacterial phenotypic characteristics, known as Biolog® system was applied. A pulsed-filed gel electrophoresis (PFGE) method for bacterial DNA fingerprinting was also evaluated and used for the identification of the inoculated bacteria. The methods described in the study were found to be effective for the identification of pathogenic bacteria from skins and effluent. Note de contenu : - MATERIALS AND METHODS : Isolation and primary identification of inoculated bacterial species - Gram-staining and spore-staining - Biochemical following standard methods - The biolog system for bacterial identification - Extraction and purification of bacterial DNA for pulsed-field gel electrophoresis (Bacillus cereux - Pseudomonas aeruginosa) - Digestion of the bacterial DNA - Electrophoresis of bacterial DNA - RESULTS AND DISCUSSION : Bacterial growth on selective media (Bacillus cereus - Pseudomonas aeruginosa) - Gram-staining and spore-staining - Biochemical tests - The biolog system for bacterial identification - Electrophoresis of bacterial DNA En ligne : https://drive.google.com/file/d/1-cjGV1x0pnaNgB2dUiGLRtm5YRBYxREf/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=17372 [article]

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Microorganisms : growth in the tannery / Anne Lama in LEATHER INTERNATIONAL, Vol. 213, N° 4816 (11-12/2011)  

[article]  inLEATHER INTERNATIONAL > Vol. 213, N° 4816 (11-12/2011) . - p. 48-49 Titre : Microorganisms : growth in the tannery Type de document : texte imprimé Auteurs : Anne Lama, Auteur ; Geoffrey E. Attenburrow, Auteur ; Margaret Bates, Auteur ; Anthony D. Covington, Auteur ; Stuart C. Allen, Auteur ; A. Paula M. Antunes, Auteur Année de publication : 2011 Article en page(s) : p. 48-49 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bactéries pathogènes Champignons pathogènes Tannage Index. décimale : 675 Technologie du cuir et de la fourrure En ligne : https://drive.google.com/file/d/1a5hZ80mfG_vGS2RTypETdu52eRv5cEri/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=12758 [article]

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Survival and growth of bacillus cereus, pseudomonas aeruginosa and staphylococcus aureus in extreme environments present during the early stages of a conventional leather making process / Anne Lama in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CVII, N° 6 (06/2012)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CVII, N° 6 (06/2012) . - p. 186-194 Titre : Survival and growth of bacillus cereus, pseudomonas aeruginosa and staphylococcus aureus in extreme environments present during the early stages of a conventional leather making process Type de document : texte imprimé Auteurs : Anne Lama, Auteur ; Stuart C. Allen, Auteur ; Geoffrey E. Attenburrow, Auteur ; Anthony D. Covington, Auteur ; A. Paula M. Antunes, Auteur Année de publication : 2012 Article en page(s) : p. 186-194 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Croissance Bactéries pathogènes Cuirs et peaux Cuirs et peaux de veaux Hypochlorite de sodium Prétannage Tannage au chrome Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Raw hides/skins used as a raw material for manufacturing leather contain various microorganisms including potential pathogens. The presence of hazardous chemicals during a conventional leather-making process may create extreme environmental conditions for the growth of the bacteria present on hides/skins. Bacteria, however, are known to survive harsh environmental conditions. This study was undertaken in order to determine the survival and growth of certain bacteria during a conventional pre-tanning and chromium-tanning process. Calfskin pieces were decontaminated and inoculated with log8 (108) to log10 (1010) colony forming units (cfu) of Bacillus cereus ATCC11778, Pseudomonas aeruginosa ATCC10145 and Staphylococcus aureus ATCC25923, and the survival was monitored following a conventional pre-tanning and chromium-tanning process. The presence of inoculated bacteria in effluent and on skins was determined. A higher bacterial growth was observed during the pre-soaking process, followed by a significant reduction (p?0.05) during the main-soaking, unhairing and reliming processes. Although limited, bacterial growth was observed during the subsequent deliming, bating, pickling and tanning processes. The study showed that these bacterial species are not only capable of surviving a conventional leather-making process, but also showed the ability to proliferate if provided with suitable environmental conditions. Note de contenu : - EXPERIMENTAL : Sodium hypochlorite treatment - Bacterial inoculation- Leather-making process - Bacterial enumeration in effluent and swabs - Sample plating - Confirmatory tests - Statistical analysis - RESULTS : Preliminary experiments - Bacterial isolation from effluent prior to and during processing of calfskins - Bacillus cereus - Pseudomonas aeruginosa - Staphylococcus aureus - Bacterial isolation from swabs En ligne : https://drive.google.com/file/d/17fvgNXtwDTNLvqW2-_BXcSKy22er_no4/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=15532 [article]

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