| Titre : |
Methods of isolation and identification of pathogenic and potential pathogenic bacteria from skins and tannery effluents |
| Type de document : |
texte imprimé |
| Auteurs : |
Anne Lama, Auteur ; Margaret Bates, Auteur ; Anthony D. Covington, Auteur ; Stuart C. Allen, Auteur ; A. Paula M. Antunes, Auteur |
| Année de publication : |
2013 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Bactéries -- Identification
Bactéries -- Isolement
Bactéries pathogènes
Cuirs et peaux -- Analyse
Eaux usées -- Analyse
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Currently there is no standard protocol available within the leather industry to isolate and identify pathogenic bacteria from hides, skins or tannery effluent. This study was therefore carried out to identify simple but effective methods for isolation and identification of bacterial pathogens from the effluent and skins during leather processing. Identification methods based on both phenotypic and genotypic characteristics were investigated. Bacillus cereus and Pseudomonas aeruginosa were used as indicator bacteria to evaluate the isolation and identification methods. Decontaminated calfskins were inoculated with a pure culture of the above mentioned bacterial species followed by pre-tanning and chromium tanning processes. Effluent samples were collected and skins were swabbed at the end of each processing stage. Bacterial identification was carried out based on the phenotypic characteristics; such as colony appearance on selective solid media, cell morphology following a standard Gram-staining and spore staining techniques, and biochemical reactions, e.g., the ability of a bacterial species to ferment particular sugars and ability to produce certain enzymes. Additionally, an identification system based on bacterial phenotypic characteristics, known as Biolog® system was applied. A pulsed-filed gel electrophoresis (PFGE) method for bacterial DNA fingerprinting was also evaluated and used for the identification of the inoculated bacteria. The methods described in the study were found to be effective for the identification of pathogenic bacteria from skins and effluent. |
| Note de contenu : |
- MATERIALS AND METHODS : Isolation and primary identification of inoculated bacterial species - Gram-staining and spore-staining - Biochemical following standard methods - The biolog system for bacterial identification - Extraction and purification of bacterial DNA for pulsed-field gel electrophoresis (Bacillus cereux - Pseudomonas aeruginosa) - Digestion of the bacterial DNA - Electrophoresis of bacterial DNA
- RESULTS AND DISCUSSION : Bacterial growth on selective media (Bacillus cereus - Pseudomonas aeruginosa) - Gram-staining and spore-staining - Biochemical tests - The biolog system for bacterial identification - Electrophoresis of bacterial DNA |
| En ligne : |
https://drive.google.com/file/d/1-cjGV1x0pnaNgB2dUiGLRtm5YRBYxREf/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=17372 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CVIII, N° 2 (02/2013)
Aller sur edunet
Accueil
Methods of isolation and identification of pathogenic and potential pathogenic bacteria from skins and tannery effluents in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CVIII, N° 2 (02/2013)
