Examination of efficiency and sufficiency of salt-pack curing method / Emel Aslan in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 95, N° 3 (05-06/2011)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 95, N° 3 (05-06/2011) . - p. 98-103 Titre : Examination of efficiency and sufficiency of salt-pack curing method Type de document : texte imprimé Auteurs : Emel Aslan, Auteur ; Meral Birbir, Auteur Année de publication : 2011 Article en page(s) : p. 98-103 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Cuirs et peaux -- Conservation Tags : Peau  'Méthodes  conservation'  'Cristaux  sel'  'Teneur  humidité'  cendres'  'Mesure  pH'  'Prolifération  bactérienne'  'Bactéries  lipolytique'  mésophiles'  protéolytiques' Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Conservation defects affect leather quality adversely and cause important economic losses. Therefore, the goal of this study was to evaluate hide quality by comparing important parameters of salt-pack cured hides and determine efficiency and sufficiency of the curing method in the areas both with and without salt crystals present on the hides. The areas with and without salt crystals on the salt-pack cured hide samples, (collected from different tanneries in the leather Organized Tannery Region, Tuzla-Istanbul-Turkey), were examined in terms of pH values, moisture and ash contents, brine saturations, total mesophile, proteolytic and lipolytic mesophile bacterial numbers. The pH values of the both areas of all the hide samples (6.3-7.4) were found to be optimum for bacterial growth. Moisture contents of the areas without salt crystals present on the hide samples (42-57 %). Ash contents and salt saturations of both types of area of all the hide samples (21-36 %) and 100 % respectively) were found to be the same as standard values. Although the hides were salt-pack cured, high numbers of mesophile, proteolytic and lipolytic mesophile bacteria were isolated from both areas of the hides. Mean values of the mesophile (2X10 7 c.f.u/g, 1.5 X 10 7 c.f.u./g). As a conclusion, due to an inadequately applied salt-pack curing process, this method was not a satisfactory method to prevent bacterial proliferation on hides. Hence, the traditional salt-pack curing method should be modified to improve the counter bacterial effect. Note de contenu : - Collection of the salt-pack cured hide samples from different tanneries - Measurement of pH values of the areas with and without salt crystals of the salt-pack cured hides - Moisture, ash and salt saturation of the areas with and without salt crystals of the salt-pack cured hides - Examination of mesophilic, proteolytic and lipolytic mesophilic bacteria at the areas with and without salt crystals of the salt-pack cured hides. En ligne : https://drive.google.com/file/d/1g_fTb0FIPiCrFlfl4DmiG47O4D8ivFD2/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=11877 [article]

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Examination of gram-negative bacteria on salt-pack cured hides / Emel Aslan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CVII, N° 4 (04/2012)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CVII, N° 4 (04/2012) . - p. 106-115 Titre : Examination of gram-negative bacteria on salt-pack cured hides Type de document : texte imprimé Auteurs : Emel Aslan, Auteur ; Meral Birbir, Auteur Année de publication : 2012 Article en page(s) : p. 106-115 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification Bactéries à gram négatif Cuirs et peaux -- Conservation Cuirs et peaux -- Séchage Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Salt-pack curing is the most widely used method to preserve hides. However, inadequately applied salt-pack curing method supports the growth of proteolytic and lipolytic bacterial and archaeal flora which may reduce the quality of leather. Therefore, to examine the efficacy of salt-pack curing method applied to hides and to determine harmful microorganisms of the hides, Gram-negative bacteria on the salt-pack cured hides were isolated and identified and their proteolytic and lipolytic activities were investigated in the present study. Salt-pack cured hides examined were collected from different tanneries in Leather Organized Tannery Region, Tuzla-Istanbul, Turkey and 40% of the hides were imported from abroad. A total of 256 Gram-negative bacterial isolates containing 21 different genera (Acinetobacter, Aeromonas, Alcaligenes, Burkholderia, Citrobacter, Comamonas, Edwardsiella, Enterobacter, Escherichia, Hafnia, Klebsiella, Mannheimia, Pasteurella, Proteus, Pseudomonas, Salmonella, Serratia, Sphingomonas, Stenotrophomonas, Vibrio and Yersinia) and 46 different bacterial species were isolated and identified from the hide samples. The percentage of proteolytic, lipolytic and both proteolytic and lipolytic Gram-negative isolates on the hides were found as 68%, 52% and 43%, respectively. The most common Gram-negative genera on the salt-pack cured hides were Enterobacter (66), Pseudomonas (59) and Vibrio (32). These isolates showed both proteolytic and lipolytic activities in the highest number on the hides. As a conclusion, the hides contained a wide variety of destructive Gram-negative bacterial species originating from different environmental sources and traditional salt-pack curing method was not sufficient to inactivate these Gram-negative bacteria. Note de contenu : - EXPERIMENTAL : Isolation and identification of species in the family of enterobacteriacecae - Isolation and identification of pseudomonas species - Determination of protease activity - Determination of lipase activity - RESULTS AND DISCUSSION : TABLE I : Frequencies of various species of gram-negative bacteria isolated from the salt-pack cured hides - TABLE II : Total numbers of proteolytic, lipolytic, both proteolytic and lipolytic genera of gram-negative bacteria isolated from the salt-pack cured hides - TABLE III : The percentages of proteolytic, lipolytic, both proteolytic and lipolytic genera of gram-negative bacteria isolated from the salt-pack cured hides En ligne : https://drive.google.com/file/d/1LBT9am2MJIGMepWw-yHE11h7jK-NhCNK/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=14684 [article]

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Examination of gram positive bacteria on salt-pack cured hides / Emel Aslan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CVI, N° 12 (12/2011)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CVI, N° 12 (12/2011) . - p. 372-380 Titre : Examination of gram positive bacteria on salt-pack cured hides Type de document : texte imprimé Auteurs : Emel Aslan, Auteur ; Meral Birbir, Auteur Année de publication : 2011 Article en page(s) : p. 372-380 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification Bactéries à gram positif Cuir -- Séchage Cuirs et peaux -- Conservation Sels Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Salt-pack curing method has been a widely used hide preservation method in worldwide. In order to evaluate the efficiency of the salt-pack curing process on the hides, Gram-positive bacteria on the hides were characterized and their proteolytic and lipolytic activities were examined. Salt-pack cured hides examined were collected from different tanneries in Leather Organized Tannery Region, Tuzla-Istanbul, Turkiye and 40% of the hides were imported from abroad. A total of 396 Gram-positive bacteria comprising from 12 different genera (Aerococcus, Aneurinibacillus, Bacillus, Brevibacillus, Enterococcus, Geobacillus, Kocuira, Lactococcus, Paenibacillus, Streptococcus, Staphylococcus and Virgibacillus) and 47 bacterial species were isolated and identified from the hides. The total numbers of proteolytic, lipolytic and both proteolytic and lipolytic isolates on the hides were found as 278, 274 and 226, respectively. The most common Gram-positive genera on the salted hides were Staphylococcus (115 isolates), Bacillus (111 isolates) and Enterococcus (75 isolates). Bacillus and Staphylococcus isolates showed both proteolytic and lipolytic activities in the highest number on the hides. The results verified that the salt-pack curing method was not efficient in preserving the raw cattle hides. As a conclusion, since the salt-pack curing method were not applied adequately, a wide variety of Gram- positive bacterial species were isolated from the salt-pack cured hides. Therefore, the salt-pack curing method should be modified to inactivate Gram-positive bacteria found on the salt-pack cured hide samples. Note de contenu : - Isolation and identification of bacillus species - Isolation and identification of staphylococcus species - Isolation and identification of streptococcus species - Determination of protease activity - Determination of lipase activity En ligne : https://drive.google.com/file/d/1KVVhLVSXGM7Y0Vq79QXH3Mnxkj_ghKxD/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=12899 [article]

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