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In vitro cultured human skin cells as alternatives to animals for skin irritancy screening in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 14, N° 6 (12/1992)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 14, N° 6 (12/1992) Titre : In vitro cultured human skin cells as alternatives to animals for skin irritancy screening Type de document : texte imprimé Année de publication : 1992 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Cellules -- Cultures et milieux de culture Etude in vitro Fibroblastes Kératinocytes Les kératinocytes sont des cellules constituant 90 % de la couche superficielle de la peau (épiderme) et des phanères (ongles, cheveux, poils, plumes, écailles). Ils synthétisent la kératine (kératinisation), une protéine fibreuse et insoluble dans l'eau, qui assure à la peau sa propriété d'imperméabilité et de protection extérieure. L'épiderme est divisé en 4 couches basées sur la morphologie des kératinocytes (de l'intérieur vers l'extérieur) : 1. stratum germinativum (couche basale à la jonction avec le derme) 2. stratum spinosum 3. stratum granulosum 4. stratum lucidum 5. stratum corneum Les kératinocytes passent progressivement de la couche basale vers les couches supérieures par différenciation cellulaire jusqu'au stratum corneum ou ils forment une couche de cellules mortes nommées squames, par apoptose. Cette couche constitue une barrière de protection et réduit la perte d'eau de l'organisme. Les kératinocytes sont en perpétuel renouvellement. Ils mettent environ 1 mois pour aller de la couche basale au stratum corneum mais ce processus peut être accéléré en cas d'hyperprolifération de kératinocyte (psoriasis). Peau Tests de toxicité Index. décimale : 668.5 Parfums et cosmétiques Résumé : In the last few years a lot of attention has been paid to the development of the in vitro models which would substitute for animals in cutaneous irritancy studies. These models explore either organ or explant cultures using freshly excised skin or serial cultures of isolated skin cells (epidermal keratinocytes or dermal fibroblasts). The organ or explant models are suitable only for short exposures of skin samples to the compounds tested and the use of it will always be restricted by the limited availability of fresh human skin. The model that uses submerged cultures of keratinocytes or fibroblasts permits the production of a large number of cells, and permits large scale toxicity screening tests with many substances, that can be applied in a broad concentration range. Since the stratum corneum is absent in conventional (submerged) keratinocyte culture systems, this model is mainly suited for testing of water soluble compounds and it is less suitable for poorly soluble compounds and for topical products consisting of complex formulations which are made of active ingredients and their vehicles. This shortcoming can be overcome by using ‘organotypic cultures’in which keratinocytes are grown at the air-liquid interface on a suitable dermal substrate. Under these conditions, the culture forms a multilayered epidermis showing an overall structure which resembles that of a native epidermis. The presence of a coherent stratum corneum layer in these cultures permits the application of potential irritants at concentrations and in formulations as applied in vivo. For the evaluation of toxicity a number of tests have already been developed: assessment of cell viability, changes in cell morphology, modulation of cell proliferation and differentiation, monitoring of membrane damage, the measurements of the uptake or incorporation of radioactive precursors, establishment of the modulation of cell metabolism, determination of the release of inflammatory mediators, etc. All these in vitro techniques are still in a state of validation as far as their predictive value for in vivo skin irritancy is concerned. DOI : 10.1111/j.1467-2494.1992.tb00058.x En ligne : https://onlinelibrary.wiley.com/doi/10.1111/j.1467-2494.1992.tb00058.x Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=26585 [article]

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Prediction of the percutaneous penetration of ultra-violet filters used in sunscreen formulations / A. C. Watkinson in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 14, N° 6 (12/1992)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 14, N° 6 (12/1992) . - p. 265–275 Titre : Prediction of the percutaneous penetration of ultra-violet filters used in sunscreen formulations Type de document : texte imprimé Auteurs : A. C. Watkinson, Auteur ; Keith R. Brain, Auteur ; Kenneth A. Walters, Auteur ; Jonathan Hadgraft, Auteur Année de publication : 1992 Article en page(s) : p. 265–275 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Absorption cutanée Modèles mathématiques Pénétration (physique) Produits antisolaires Index. décimale : 668.5 Parfums et cosmétiques Résumé : The application of a mathematical model to estimate the extent of transdermal absorption of UV-filters commonly used in sunscreen formulations is described. Percutaneous penetration is not a factor that has been properly addressed in the literature and the penetration/time profiles generated here suggest that significant amounts of certain of these compounds may penetrate the skin and enter the systemic circulation. The results presented indicate that further research in this area is necessary and the authors suggest that in vitro experiments with human skin are conducted with both current and new UV filters to quantify the degree of dermal penetration of these substances. DOI : 10.1111/j.1467-2494.1992.tb00059.x En ligne : https://onlinelibrary.wiley.com/doi/10.1111/j.1467-2494.1992.tb00059.x Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=26586 [article]

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In-use tolerability of two new syndet bars with sodium cocoyl isethionate, disodium lauryl sulfosuccinate and wheat starch as major ingredients in differing relations: results of a cross-over trial / H. C. Korting in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 14, N° 6 (12/1992)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 14, N° 6 (12/1992) . - p. 277–282 Titre : In-use tolerability of two new syndet bars with sodium cocoyl isethionate, disodium lauryl sulfosuccinate and wheat starch as major ingredients in differing relations: results of a cross-over trial Type de document : texte imprimé Auteurs : H. C. Korting, Auteur ; A. Lukacs, Auteur ; D. Hamm, Auteur ; Klaus Stanzl, Auteur ; O. Braun-Falco, Auteur Année de publication : 1992 Article en page(s) : p. 277–282 Note générale : Bibliogr. Langues : Anglais (eng) Tags : 'Propriétés  en  cours  d'utilisation'  'Barres  de  syndet'  Tolérabilité  'Cocoyl  iséthionate  sodium'  'Disodium  laurylsulfosuccinate'  'Amidon  blé' Index. décimale : 668.5 Parfums et cosmétiques Résumé : In a cross-over trial two different new syndet bars differing in their content of sodium cocoyl isethionate, disodium lauryl sulfosuccinate and wheat starch as major components were compared in the cleansing of hand skin of 72 healthy volunteers. The preparation designated ‘T’containing a higher amount of wheat starch turned out to be less irritant as judged from the parameters itching and erythema (p < 0.05, Wilcoxon's test for matched pairs). The present trial design looks appropriate to differentiate the irritancy potential of two chemically similar syndet bar preparations. DOI : 10.1111/j.1467-2494.1992.tb00060.x En ligne : https://onlinelibrary.wiley.com/doi/10.1111/j.1467-2494.1992.tb00060.x Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=26587 [article]

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