Monitoring the mode of action of synthetic and natural biocides against Aeromonas hydrophila by Raman spectroscopy and chemometrics / Megha Mehta in JOURNAL OF LEATHER SCIENCE AND ENGINEERING, Vol. 3 (Année 2021)  

[article]  inJOURNAL OF LEATHER SCIENCE AND ENGINEERING > Vol. 3 (Année 2021) . - 15 p. Titre : Monitoring the mode of action of synthetic and natural biocides against Aeromonas hydrophila by Raman spectroscopy and chemometrics Type de document : texte imprimé Auteurs : Megha Mehta, Auteur ; Yang Liu, Auteur ; Mark Waterland, Auteur ; Geoff Holmes, Auteur Année de publication : 2021 Article en page(s) : 15 p. Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Aeromonas hydrophila Analyse multivariée Antimicrobiens Chimiométrie Cuirs et peaux -- Conservation Huile essentielle d'origan Inhibiteurs (chimie) Spectroscopie Raman Thiocyano-méthylthiobenzotiazole Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : We have investigated the mode of action of synthetic biocides, (2-(thiocyanomethylthio) benzothiazole(TCMTB), dichlorophen, (commonly used in leather industry for preservation) and natural biocides, oregano and eucalyptus oils, on Aeromonas hydrophila using Raman spectroscopy in collaboration with multivariate analysis and 2D correlation spectroscopy to evaluate whether Raman spectra acquired contained valuable information to study the action of biocides on bacterial cells. The growth of A. hydrophila in clear and outer edge zone of inhibition differ in their reaction with different biocides, which allows us to highlight the differences as a characteristic of two kinds of bacteria. Such classification helps identify oregano oil as the most effective biocide by altering clear and outer edge zone of bacteria. Standard disk diffusion assay method was used for screening biocide bacteria interactions and later analysed by Raman spectroscopy. The paper also presents the introduction of TCMTB and oregano oil into leather processing stages to examine and determine the antimicrobial effect as an application to real-world setting. Therefore, we conclude that Raman spectroscopy with appropriate computational tools constitutes a powerful approach for screening biocides, which provide solutions to all the industries using biocides including leather industry, considering the potentially harmful effect of biocides to humans and the environment. Note de contenu : - MATERIALS AND METHODS : Biocides and bacteria - Biocides, bacteria and leather treatment - Data acquisition and Raman spectral processing - RESULTS AND DISCUSSION : Disk diffusion agar plate test - Raman spectroscopy identifies biomolecular changes due to biocide treatment - Multivariate analysis - Two-dimensional correlation spectroscopy (2D-COS) - Antimicrobial effect of leather treated with biocides - Table 1 : Disk diffusion determined zones of inhibition (mm) for A. hydrophila strain after treatment with TCMTB, DCP, oregano oil and eucalyptus oil DOI : https://doi.org/10.1186/s42825-021-00062-3 En ligne : https://link.springer.com/content/pdf/10.1186/s42825-021-00062-3.pdf Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37546 [article]

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