A rapid method for measuring elastin degradation and its application in leather manufacturing / Xu Zhang in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXV, N° 8 (08/2020)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXV, N° 8 (08/2020) . - p. 294-300 Titre : A rapid method for measuring elastin degradation and its application in leather manufacturing Type de document : texte imprimé Auteurs : Xu Zhang, Auteur ; Mengchu Gao, Auteur ; Chunxiao Zhang, Auteur ; Sadaqat Ali Chattha, Auteur ; Biyu Peng, Auteur Année de publication : 2020 Article en page(s) : p. 294-300 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Chromatographie en phase liquide à hautes performances Confitage Le confitage est une action biochimique effectuée au moyen de produits enzymatiques, qui a pour but de dégrader les fibres élastiques, contribuant ainsi à augmenter la souplesse du cuir. En outre, les enzymes complètent la dégradation des résidus épidermiques, donnant ainsi une fleur plus propre et plus lisse. Desmosine La desmosine est une structure moléculaire formée par l'association de quatre chaînes latérales de lysine. On la retrouve associée à l'élastine où elle forme des interconnexions (liaisons covalentes) entre les différentes fibres et participe ainsi à ses propriétés mécaniques. (Wikipedia) Elastine -- Détérioration Liqueurs de tannage Tannage au chrome Teneur en desmosine Wet-blue (tannage) Peau tannée au chrome (le chrome donne une couleur bleue) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The degradation of elastin in skins during leather manufacturing can increase the yield area, softness and flatness of the leather, but, excessive degradation of elastin in leather processing induces looseness and increases veininess in the final leather product. However, the characterization of the degradation degree of elastin in skins and leathers was mostly studied through histological analysis qualitatively. There is an urgent need to develop a more efficient quantitative analytical strategy to evaluate the degradation of elastin in leather making processes. In this study, a simple and rapid HPLC method is developed for measuring elastin degradation in skins, leathers and leather processing liquors through determining the biological markers of elastin, namely desmosines. The separation of analytes was conducted on an C18 column (4.6 × 150 mm, 4.0 μm) at 30 ℃; the wavelength of diode array detector (DAD) was set at 275 nm; the mobile phases were composed of methanol and aqueous acetic acid (2.0 %, v/v). A gradient elution was carried out at a flow rate of 0.5 mL/min. It has been witnessed that Cr (III) has no effect on the retention time and peak area of desmosines, when the concentration of Cr (III) was in the range of 0 to 200 mg/L. In quantitative analysis, all of the calibration curves showed good linear regression (R2 ≥ 0.9990) within the tested ranges, and the recovery of desmosines was 100.9 % and 102.6 % for elastin hydrolysate and Cr-tanned elastin hydrolysate, respectively. The content of desmosines in elastin fiber, Cr-tanned elastin fiber and leather manufacturing liquors measured by established HPLC-DAD were comparable to the results obtained by amino acid analyzer. In the wet blue bating process, the quantitative analysis results are consistent with the histological staining results. The results demonstrate that the developed method is accurate and effective and can be readily utilized for the comprehensive process control of leather manufacturing. Note de contenu : - MATERIALS AND METHODS : Chemicals and reagents - Standard solutions - Preparation of samples - HPLC-DAD conditions - Method validation - Wet blue bating process - Determination of desmosines by amino acid analyzer - RESULTS AND DISCUSSION : Optimization of HPLC conditions - Effect of Cr(III) on des quantitation - Method validation - Determining contents of desmosines in varying leathe processing liquors - Wet blue bating process DOI : https://doi.org/10.34314/jalca.v115i8.3842 En ligne : https://drive.google.com/file/d/1wYegzMOMz23n_3RRtHL5xozXaGO0kzXm/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=34436 [article]

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