Culture conditions optimisation of bacillus subtilis for producing bioflocculant / Changqing Zhao in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 104, N° 3 (05-06/2020)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 104, N° 3 (05-06/2020) . - p. 151-155 Titre : Culture conditions optimisation of bacillus subtilis for producing bioflocculant Type de document : texte imprimé Auteurs : Changqing Zhao, Auteur ; Zhang Yizhuo, Auteur ; Yang Qinhuan, Auteur ; Yang Yang, Auteur Année de publication : 2020 Article en page(s) : p. 151-155 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bacillus subtilis Bactéries -- Culture et milieux de culture Biofloculant Eaux usées -- Décontamination Eaux usées -- Epuration Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : A bioflocculant seen as an environmentally friendly flocculant with potential for development, with the capacity to improve its activity and yield has become a hot topic of research among scholars in various countries. This study found that a bioflocculant produced by Bacillus subtils is able to treat leather wastewater, but the flocculation activity of the bioflocculant needs to be improved. In this paper, the culture conditions of Bacillus subtilis were optimised by single-factor and orthogonal tests. The results showed that the bioflocculant produced by Bacillus subtilis through optimising the culture conditions had good flocculation efficiency. The optimal culture temperature after optimisation was 36°C, the inoculum dose was 5% (v/v), at pH7.5, the culture time was 48 hours, the flocculation efficiency increased from 46.12% to 73.71%, an increase of 59.82%, and the production of flocculant increased from 0.45g/L to 0.61g/L, an increase of 35.56%. Through optimising the culture conditiops of Bacillus subtilis the flocculation efficiency of the bioflocculant, was improved and both the cost of flocculation and secondary pollution were reduced. Note de contenu : - EXPERIMENTAL PROCEDURE : Materials - Activation of Bacillus subtilis species - Initial culture of Bacillus subtilis - Collection of bioflocculant - SINGLE FACTOR OPTIMISATION OF CULTURE CONDITIONS : Determination of culture temperature - Determination of inoculum dose - Determination of culture pH - Determination of culture time - ORTHOGONAL OPTIMISATION OF CULTURE CONDITIONS - RESULTS AND DISCUSSION : Effect of culture temperature - Determination of optimum inoculum dose - Determination of optimal pH - Determination of optimum culture time - Orthogonal test of culture conditions - Comparison of effects before and after optimisation of culture conditions En ligne : https://drive.google.com/file/d/1ypFkJYJntpsnfPxiGliF-VWD9zQ9BMcj/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=34245 [article]

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