| Titre : |
Synthesis of novel tetracationic asymmetric monomeric monomethine cyanine dyes – highly fluorescent dsDNA probes |
| Type de document : |
texte imprimé |
| Auteurs : |
Aleksey Vasilev, Auteur ; Todor Deligeorgiev, Auteur ; Stefka Kaloyanova, Auteur ; Stanimir Stoyanov, Auteur ; Vera Maximova, Auteur ; Juan J. Vaquero, Auteur ; Julio Alvarez-Buila, Auteur |
| Année de publication : |
2011 |
| Article en page(s) : |
p. 69-74 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
ADN
Cyanines monométhines
Fluorescence
Lumière -- Absorption
Pigments à effets spéciaux -- Effets de la lumière
|
| Index. décimale : |
667.3 Teinture et impression des tissus |
| Résumé : |
Quaternisation of monomethine cyanine dyes bearing haloalkyl chains, with the appropriate monoquaternised 1,4-diazabicyclo[2.2.2]octane derivatives, yielded novel tetracationic asymmetric monomeric monomethine cyanine dyes belonging to the thiazole orange family. The optimal conditions for this reaction were investigated. The longest wavelength absorption maxima of the studied dyes are in the region 504–507 nm. The molar absorptivities of the target dyes are high, with values between 70 500 and 99 500 l mol?1 cm?1. The fluorescence intensity and the fluorescence quantum yields of the free dyes in solution or in the presence of double-stranded DNA were determined using thiazole orange for comparison. The dyes are suitable for nucleic acid detection and have a high binding affinity, attributable to their four positive charges. |
| DOI : |
10.1111/j.1478-4408.2010.00280.x |
| En ligne : |
http://onlinelibrary.wiley.com/doi/10.1111/j.1478-4408.2010.00280.x/pdf |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10906 |
in COLORATION TECHNOLOGY > Vol. 127, N° 1 (2011) . - p. 69-74
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