Basement membrane damage, a sign of early aginig, and laminin 5, a key player in basement membrane care / Satoshi Amano in IFSCC MAGAZINE, Vol. 3, N° 4 (10-11-12/2000)

[article]  inIFSCC MAGAZINE > Vol. 3, N° 4 (10-11-12/2000) . - p. 15-23 Titre : Basement membrane damage, a sign of early aginig, and laminin 5, a key player in basement membrane care Type de document : texte imprimé Auteurs : Satoshi Amano, Auteur ; Yukiko Matsunaga, Auteur ; Nobuko Akutsu, Auteur ; Kuniko Kadoya, Auteur ; Minoru Fukuda, Auteur ; Izumi Horii, Auteur ; Tasuku Takamatsu, Auteur ; Eijiro Adachi, Auteur ; Toshio Nishiyama, Auteur Année de publication : 2000 Article en page(s) : p. 15-23 Note générale : Bibliogr. Langues : Anglais (eng) Index. décimale : 668.5 Parfums et cosmétiques Résumé : Basement membrane (BM) at the dermalepidermal (D-E) junction plays important roles in maintaining the structure and function of skin. However, little is known about the ultrastructural histology and morphology of BM in young skin. We have found that BM structure even in 30-year-old female skin was alterde and showed disruption and reduplication, while such alternations were scarcely present in skin from subjects in the early 20s. This is consistent with the known changes in skin surface texture in the late 20s and early 30s. Such damage and alteration are thought to be a foremost sign of early aging of skin, since altered BM structure, which may be a result of accumulation of repeated damage during normal daily life, is frequently found in aged people. Therefore, early care of the BM is important to prevent skin aging. BM has a complex anchoring structure involving many components, among which laminin 5 is a key component. Laminin 5 has bifunctional domains which can bind integrins of basal keratinocytes and type VII collagen, forming anchoring fibrils. We found that purified laminin 5 accelerated the formation of BM at the D-E junction in a skin-equivalent model. Furthermore, we found that soybean lysophospholipids stimulate laminin 5 production by human cultured keratinocytes, by using a novel laminin 5-specific sandwich ELISA method. An increase of laminin 5 mRNA levels was also confirmed by the TaqMan PCR method. In the lysophospholipid-treated skin-equivalent model, increased deposition of laminin 5 and type IV collagen and more continuous BM were observed at the D-E junction. These results suggest that increased laminin 5 production in epidermal cells enhances the repair of damaged BM at the D-E junction of skin, and this may be an effective approach to prevent skin aging. Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10791 [article]

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Development of self-dissolving microneedles consisting of hyaluronic acid as an anti-wrinkle treatment / Yukiko Matsunaga in IFSCC MAGAZINE, Vol. 15, N° 2 (04-05-06/2012)

[article]  inIFSCC MAGAZINE > Vol. 15, N° 2 (04-05-06/2012) . - p. 73-78 Titre : Development of self-dissolving microneedles consisting of hyaluronic acid as an anti-wrinkle treatment Type de document : document électronique Auteurs : Yukiko Matsunaga, Auteur ; Shigeyoshi Fujiwara, Auteur ; Yuichiro Mori, Auteur ; Akiko Miyake, Auteur ; Haruyo Yamanishi, Auteur ; Madoka Kage, Auteur ; Yoshihiro Tokudome, Auteur ; Fumie Hashimoto, Auteur ; Takeshi Hariya, Auteur Année de publication : 2012 Article en page(s) : p. 73-78 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Antiâge:Antirides Cosmétiques Hyaluronique, acide L'acide hyaluronique est un type de polysaccharide (plus précisément une glycosaminoglycane) non fixé à une protéine centrale et largement réparti parmi les tissus conjonctifs, épithéliaux et nerveux animaux. Il se trouve notamment dans l'humeur vitrée et le liquide synovial. Il est l'un des principaux composants de la matrice extracellulaire ainsi que de certaines mucoprotéines lorsqu'il est associé à une fraction protéique. Depuis les années 1990, il est très utilisé dans divers dispositifs médicaux, médicamenteux et cosmétiques (présenté dans ce dernier cas comme "antistatique, humectant, hydratant, conditionneur cutané, anti-âge, etc."), bénéficiant d'un effet de mode grandement soutenu par une large publicité commerciale. Contribuant de façon significative à la prolifération et à la migration des cellules, l'acide hyaluronique est aussi impliqué dans la progression de certains cancers. (Wikipedia) Index. décimale : 668.5 Parfums et cosmétiques Résumé : Hyaluronic acid, a nonsulfated glycosaminoglycans, plays a key role in water retention in the extracellular matrix. Quantitative deficiency of hyaluronic acid in the skin promotes the signs of aging and therefore supplementation of hyaluronic acid is essential to facilitate skin rejuvenation. Unfortunately, hyaluronic acid does not readily enter cutaneous tissue by passive transport because of its high molecular weight. However, self-dissolving microneedles, which are needle-shaped structures designed to pierce the stratum corneum, can be used to deliver agents to the stratum corneum or epidermis and should be able to deliver hyaluronic acid. In this study, we developed self-dissolving microneedles composed of hyaluronic acid for cosmetic use. Microscopic examination demonstrated that the tip of these hyaluronic acid microneedles reached the lower layer of the stratum corneum. Addition of hyaluronic acid to keratinocytes promoted celle proliferation and induced upregulation of profilaggrin gene expression. Hyaluronic acid synthase 1 mRNA was also upregulated by addition of hyaluronic aid to fibroblasts. Successive applications of self-dissolving microneedles to crow's feet for 4 weeks resulted in significant improvements in wrinkle formation and skin elasticity. These findings suggest that introduction of hyaluronic acid into the stratum corneum with microneedles may be an effective approach for cosmetic improvement of wrinkles. Self-dissolving microneedles are also expected to be effective for transdermal delivery of other cosmetically active agents. Note de contenu : - EXPERIMENTAL : Materials - Microscopic study - Cell culture and real-time quantitative PCR - Evaluation of self-dissolving microneedles as an anti-wrinkle agent - Statistical analysis - RESULTS AND DISCUSSION : Overview of microneedles - Microneedles penetrate the stratum corneum - Hyalunonic acid promotes proliferation and induces upregulation of profilaggrin in keratinocytes - Hyaluronic acid synthetase 1 mRNA is upregulated by hyaluronic acid addition in fibroblasts - Successive applications of self-dissolving microneedles markedly improves wrinkles Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=14955 [article]

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