Exploring DNA diversity in leathers : an approach on identification of origin / N. Pavithra in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVI, N° 1 (01/2021)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVI, N° 1 (01/2021) . - p. 22-29 Titre : Exploring DNA diversity in leathers : an approach on identification of origin Type de document : texte imprimé Auteurs : N. Pavithra, Auteur ; A. B. Aishwarya, Auteur ; A. Sahaya Pravin, Auteur Année de publication : 2021 Article en page(s) : p. 22-29 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bovins -- Races Caractérisation Cuirs et peaux -- Identification Génétique animale Marqueurs génétiques Moutons -- Races Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : DNA based approaches have become widespread in recent times to identify the origin of samples when its phenotypic characteristics are not distinguishable. This, in particular, applies to the leather industry wherein with an increase in duplicated embossing of grain patterns; there is a need to detect the animal origin of commercial leather articles. Thus, the characterization of molecular markers that enables rapid detection of the leather source helps us in precise species identification. The present study aims to generate definite sequences between the four major species in the Bovidae family (Buffalo, Cow, Goat, and Sheep), which are the major players in the manufacture of leather products, especially in India. Based on specific mitochondrial sequences, a specific fragment of the mitochondrial 12SrRNA gene was amplified by PCR as a marker for species-level identification. By the maximum homogeneity, from the NCBI and BOLD database, the BLAST analysis of the sequences of amplicons from unknown sources, distinguish closely related species of the subfamilies Bovinae (buffalo and Cow) and Caprinae (sheep and goat) and this 12SrRNA based PCR-BLAST analysis is a good tool to identify the origin and control the quality of leathers that are being manufactured. The present study has optimized an approach for the extraction and amplification of DNA from the finished leather, which is one of the most significant challenges because of the vigorous processes encountered during their manufacture. The findings of the study have commercial value at large scale. Note de contenu : - EXPERIMENTAL : Sample collection - DNA extraction from leather samples - Primer design - PCR amplification of control and test samples - DNA sequencing - Authentication of the method - BIOINFORMATICS ANALYSIS : Identification using BLAST - RESULTS AND DISCUSSION : Morphological analysis - DNA extraction from finished leather samples - PCR amplification and DNA sequencing of control and test samples - Sequence submission to genbank database - Table 1 : Comparison on thickness profile of the animal skin of the bovidae family until tanning and after finishing - Table 2 : Details on primers of specific region chosen for the animals of bovidae family for identification DOI : https://doi.org/10.34314/jalca.v116i1.4217 En ligne : https://drive.google.com/file/d/1cvgViM5kasSLp2eD9KxlbD_R8O7avJ8x/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=35118 [article]

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Identification des espèces par analyse ADN / Anne-Laure Hans in CTC ENTREPRISES, N° 4 (2008)  

[article]  inCTC ENTREPRISES > N° 4 (2008) . - p. 10-12 Titre : Identification des espèces par analyse ADN Type de document : texte imprimé Auteurs : Anne-Laure Hans, Auteur Année de publication : 2008 Article en page(s) : p. 10-12 Langues : Français (fre) Catégories : ADN Analyse génétique Cuirs et peaux -- Identification Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : CTC souhaite développer de nouvelles méthodes d'identification de cuirs. Possédant déjà une base de données photographiques et d'un savoir-faire reconnu, CTC a étudié les nouvelles technologies utilisables dans l'histologie des peaux et notamment la génétique. Note de contenu : - Contexte - Objectifs - Technique d'extraction et d'analyse : Préparation du cuir - Lyse de la poudre obtenue - Extraction de l'ADN - Amplification de l'extrait pour analyser son ADN - Résultats obtenus - Perspectives En ligne : https://drive.google.com/file/d/1K__YW0qrxs_TRMIaaShdhoZldfolplmO/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39405 [article]

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Leather species identification via mitochondrial DNA polymerase chain reaction / Xiang Zhang in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIV, N° 7 (07/2019)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIV, N° 7 (07/2019) . - p. 265-270 Titre : Leather species identification via mitochondrial DNA polymerase chain reaction Type de document : texte imprimé Auteurs : Xiang Zhang, Auteur ; Steve Lange, Auteur Année de publication : 2019 Article en page(s) : p. 265-270 Note générale : Bibliogr. Langues : Américain (ame) Catégories : ADN mitochondrial Cuirs et peaux -- Analyse Cuirs et peaux -- Identification Génétique animale Réaction en chaîne par polymérase Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Currently the identification of leather species is mainly via a skilled operator using optical microscopy and an extensive visual reference library. However, due to the limitations of optical microscopy and in cases where the leather surface is heavily corrected or finished, it is not always possible to accurately determine the leather species. Therefore, it is crucial to develop a reliable and easy to perform approach to overcome these limitations. In this paper, we used the unique DNA sequence present in every species to identify leather. The conversion process of making leather destroys much of the DNA present but the high copy number of mitochondrial DNA (mtDNA) in relation to nuclear DNA makes some mtDNA likely to survive and be detected via polymerase chain reaction (PCR). However, leather DNA is highly degraded and inhibitors present in the extracted DNA make successful PCR challenging. There are only a few related works reported in the literature attempting to use DNA for leather species identification and they provide limited technical details. In this study, species-specific primers for PCR were designed, conditions for DNA extraction from leather followed by PCR were optimized, and a detailed protocol was also provided. As a proof of concept, our approach demonstrated that the species of leather can be reliably detected via PCR targeting mtDNA. Note de contenu : - EXPERIMENTAL : Reference DNA - Leather DNA extraction - mtDNA sequence analysis and primer design - Leather species identification via mtDNA PCR - PCR product analysis via agarose gel electrophoresis - RESULTS AND DISCUSSION : Reference genomic DNA preparation - Leather DNA extraction - Species-specific primer design - Optimization of PCR condition En ligne : https://drive.google.com/file/d/1LSX8z8UCK5XOkjgexJmC7lyWH6zZR4KQ/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=32785 [article]

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