[article]
| Titre : |
Lipid analysis and transepidermal water loss in snakes |
| Type de document : |
texte imprimé |
| Auteurs : |
Nicole Widler, Auteur ; Andreas Sigrist, Auteur ; Frank M. Gafner, Auteur |
| Année de publication : |
2002 |
| Article en page(s) : |
p. 24-29 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Tags : |
Analysis Aridity 'Skin lipids' Reptiles Snakes 'Transepidermal water loss' |
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
The lipids of 101 snake sheddings from 32 different species kept at Pentapharm's serpentarium were extracted and analized by HPLC/LSD (light scattering detection) and TLC. The snake shadding extracts were compared with those of human stratum corneum. As revealed by HPLC/LSD analysis, the lipids from the various snake species and from humans are qualitatively not very different. The greatest differences could be observed quantitatively in the neutral lipids such as cholesterol. Main components are cholesterol, fatty acids, triglycerides, glycoceramides and phospholipids. The TLC analysis indicated that the extracts differ above all in the composition of neutral lipids, in contrast to polar lipids that do not show many differences. The transepidermal water loss (TEWL) of 19 different snake species was measured for the first time, according to our knowledge, directly in-vivo using a Tewameter. The results show that undisturbed snakes have a considerably lower TEWL than a stressed common lancehead ( Bothrops atrox). The results of the lipid analysis as well as the direct in-vivo TEWL measurements did not allow confirmation of the thesis of an inverse correlation between the TEWL of a snake and the aridity of its habitat.
The HPLC/LSD analysis of human stratum corneum showed a very similar profile a the chromatograms of the snake sheddings. Our analysis did not allow for differentiation between the various classes of ceramides and other lipids that may account for the differences in percutaneous absorption that have been reported for snake and human skin. But on a group-classification basis, snake sheddings should be taken into account as a simple stratum corneum model considering the ease of appendageal penetration pathways have to be kept in mind. A similar esterase activity in snake sheddings and human skin was described previously. |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10663 |
in IFSCC MAGAZINE > Vol. 5, N° 1 (01-02-03/2002) . - p. 24-29
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