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Use of dendritic cells for the identification of skin allergens / Nicola Gilmour in IFSCC MAGAZINE, Vol. 6, N° 4 (10-11-12/2003)
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Titre : Use of dendritic cells for the identification of skin allergens Type de document : texte imprimé Auteurs : Nicola Gilmour, Auteur ; D. A. Basketter, Auteur Année de publication : 2003 Article en page(s) : p. 287-293 Note générale : Bibliogr. Langues : Anglais (eng) Tags : 'Contact allergène 'Cellules dendritiques' 'Techniques in-vitro' de Langerhans' 'Sensibilisation alternatives' peau' Index. décimale : 668.5 Parfums et cosmétiques Résumé : There are several factors that govern the induction of allergic contact dermatitis, including skin penetration, metabolism/protein reactivity, antigen uptake and activation of Langerhans cells/dendritic cells and their migration to the lymph node to stimulate T-cells and initiate an immunological response. Dendritic cells thus represent a key cell type for the development of in-vitro cell based alternatives for the detection of contact allergens. Dendritic cells cultures exposed to the strong contact allergen 2,4-dinitrochlorobenzene(DNCB) have shown up-regulation of certain markers,although with variability and not always with evidence of specificity. We aimed to derive a purified dendritic cell population (CD11c+ cells), a cell type more relevant for the identification of skin allergens. Pure populations of dendritic cells were isolated from human peripheral blood using magnetic bead separation. The cells were cultured for 5 days in a combination of granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-4(IL-4) and transforming growth factor-beta ( TGF-beta), to generate 'Langerhans cell-life'cells. This approach produced a population that was 95% CD1a positive as determined by flow cytometry. The cells expressed human leukocyte antigen (HLA)-DR but not cluster of differentiation (CD)-86 or CD83 antigens, demonstrated endocytic ability ( fluorescein isothiocyanate-dextran uptake), were weak stimulators of the mixed lymphocyte reaction and thus could be regarded as an 'immature' Langerhans cell phenotype. Exposure of these cells to sub-toxic doses of 2,4-dinitrochlorobenzene (as determined by the XTT assay) resulted in elevated expression of HLA-DR (2 to 7-fold increase in mean fluorescence intensity [MFI]) and CD86 (15 to 20-fold increas in MFI) compared to control cells. Concurrent treatment with sub toxic doses of the irritant sodium lauryl sulphate or 0.1% dimethylsulfoxide (vehicle control) did not induce up-regulation of HLA-DR or CD86. Culture of blood derived CD11c+ dendritic cells thus may provide a population of Langerhans-like cells for the in-vitro evaluation of potential skin sensitizers. Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10547
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