A combination of three antioxidants decreases the impact of rural particulate pollution in Normal human keratinocytes / Angelica Ortiz in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 6 (12/2023)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 6 (12/2023) . - p. 791-801 Titre : A combination of three antioxidants decreases the impact of rural particulate pollution in Normal human keratinocytes Type de document : texte imprimé Auteurs : Angelica Ortiz, Auteur ; Hong Sun, Auteur ; Thomas Kluz, Auteur ; Mary S. Matsui, Auteur ; Tiffany Carle, Auteur ; David Gan, Auteur ; Terry Gordon, Auteur Année de publication : 2023 Article en page(s) : p. 791-801 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Antioxydants Cosmétiques Dermo-cosmétologie Kératinocytes Les kératinocytes sont des cellules constituant 90 % de la couche superficielle de la peau (épiderme) et des phanères (ongles, cheveux, poils, plumes, écailles). Ils synthétisent la kératine (kératinisation), une protéine fibreuse et insoluble dans l'eau, qui assure à la peau sa propriété d'imperméabilité et de protection extérieure. L'épiderme est divisé en 4 couches basées sur la morphologie des kératinocytes (de l'intérieur vers l'extérieur) : 1. stratum germinativum (couche basale à la jonction avec le derme) 2. stratum spinosum 3. stratum granulosum 4. stratum lucidum 5. stratum corneum Les kératinocytes passent progressivement de la couche basale vers les couches supérieures par différenciation cellulaire jusqu'au stratum corneum ou ils forment une couche de cellules mortes nommées squames, par apoptose. Cette couche constitue une barrière de protection et réduit la perte d'eau de l'organisme. Les kératinocytes sont en perpétuel renouvellement. Ils mettent environ 1 mois pour aller de la couche basale au stratum corneum mais ce processus peut être accéléré en cas d'hyperprolifération de kératinocyte (psoriasis). Particules fines Peau -- Effets de la pollution atmosphérique Peau -- Soins et hygiène Stress oxydatif Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : It is well established that exposure of human skin to airborne pollution, particularly in the form of particulate matter sized 2.5 μm (PM2.5), is associated with oxidative stress, DNA damage and inflammation, leading to premature signs of skin aging. Because much of the damage results from oxidative stress, we examined the effects of a topical composition containing three antioxidants in an in vitro model system to assess the potential for amelioration of premature aging. The use of multiple antioxidants was of interest based on the typical composition of therapeutic skincare products. It is important to determine the efficacy of multiple antioxidants together and develop a short-term assay for larger scale efficacy testing. - Methods : Normal human epidermal keratinocytes were exposed to a rural-derived source of PM2.5 in the presence and absence of an antioxidant mixture of resveratrol, niacinamide and GHK peptide. Endpoints related to inflammation, premature aging and carcinogenicity were monitored after 5 h of exposure and included IL-6, CXCL10, MMP-1 and NRF2. Differentially expressed genes were monitored by RNA-seq. - Results : Pre-treatment of keratinocytes with the antioxidant preparation in the absence of PM2.5 reduced baseline levels of MMP-1, IL-6 and CYP1A1 and reduced PM2.5-induced increases in all four endpoints, MMP-1, IL-6, CXCL10 and CYP1A1. Antioxidants significantly increased NRF2 protein in the presence of PM2.5, indicating a protective response. RNA-seq interrogation of antioxidant-treated cells further showed increased expression of NRF2 inducible genes. The expression of CYP1A1 and genes related to aryl hydrocarbon activation were induced by PM2.5 and suppressed by antioxidants. - Conclusions : Specific signalling pathways known to be correlated with skin inflammation and aging were examined based on their suitability for use in efficacy testing for the prevention of skin damage due to ambient hydrocarbon pollution. Endpoints examined after only 5 h of exposure provide a useful method amenable to high through-put screening. The results obtained reinforce the concept that a multiple antioxidant preparation, topically applied, may reduce pro-inflammatory signalling and cellular damage and thereby reduce premature skin aging due to exposure to rural-derived airborne pollution. Note de contenu : - MATERIALS AND METHODS : Cell culture - Exposure to rural PM Antioxidants - Cell viability - Assessment of oxidative stress, reactive oxygen species - IL-6 and MMP-1 measured by ELISA assays - NRF2 protein expression levels measured by Western blot - CYP1A1 and CXCL10 (IP-10) measured by RT-PCR analysis - RNA sequencing analysis of differentially expressed genes - Data analysis - RESULTS : Determination of optimal noncytotoxic exposure conditions for PM2.5 and AOx - IL-6 protein release by NHEK - Induction of CXCL10 mRNA by PM2.5 and inhibition by AOx in NHEK - NHEK expression of CYP1A1 mRNA - MMP-1 protein release by NHEK - Induction of NRF2 protein - RNA sequencing analysis identified DEGS En ligne : https://drive.google.com/file/d/1CshhBS3Ys2LrFsxeukcuSlS5LYn15BVZ/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40265 [article]

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Improving cellular function through modulation of energy metabolism / D. Maes in IFSCC MAGAZINE, Vol. 7, N° 2 (04-05-06/2004)

[article]  inIFSCC MAGAZINE > Vol. 7, N° 2 (04-05-06/2004) . - p. 121-126 Titre : Improving cellular function through modulation of energy metabolism Type de document : document électronique Auteurs : D. Maes, Auteur ; Donald Collins, Auteur ; Lieve Declercq, Auteur ; Reyhaned Foyouzi-Youssefi, Auteur ; David Gan, Auteur ; Thomas Mammone, Auteur ; Edward Pelle, Auteur ; Ken Marenus, Auteur ; Harvey Gedeon, Auteur Année de publication : 2004 Article en page(s) : p. 121-126 Note générale : Bibliogr. Langues : Anglais (eng) Tags : 'Adénosine  triphosphate'  Energie  'Métabolisme  énergétique'  Mitochondrie  Senescence  'exposition  aux  UV' Index. décimale : 668.5 Parfums et cosmétiques Résumé : The ambivalent consequences of mitochondrial stimulation on cellular activity have been well established. Mitochondria supply the cell with energy through a process of oxidative phosphorylation but thereby generate free radicals, resulting in the accumulation of hydrogen peroxide in the cytoplasm. We have investigated the impact of cellular senescence as well as UV irradiation, on the balance between these two activities. The adenosine triphosphate (ATP) level, DNA and protein synthesis in fibroblasts obtained from donors between 30 and 90 years of age appeared to be significantly influenced by the aging process. Both DNA and protein synthesis could be stimulated by increasing intracellular ATP levels. In-vitro senescent fibroblasts showed a reduction in the level of ATP as well as a shift in mitochondrial membrane potential. At the same time, there was an increase in intracellular hydrogen peroxide with increasing population doubling, indicating a clear dysfunction of the metabolic machinery in the mitochondria of senescent cells. To counteract this degradation of the energy pool, we treated cells with creatine, which is known to restore the pool of phosphocreatine in the mitochondria. Creatine treatment significantly increased cell survival after UV exposure, stimulated the repair of UVB-induced DNA damage in keratinocytes and caused a significant reduction in the number of sunburn cells in a UVB-exposed reconstituted skin model. These results clearly indicate that restoration of the energy pool in mitochondria increased cellular self-defense mechanism. These data show the important role played by the mitochondrial energy metabolism on the aging process, and indicate a possible therapy that can be used to counteract this negative effect. Treatment with creatine seems to provide the necessary boost to the cellular metabolism, which leads to an induction of a significant amount of protection and repair to human skin cells. Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10526 [article]

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