Antibacterial properties of several lichen extracts against two moderately halophilic bacteria from salted sheepskins / Ipek Türkmenoglu in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVII, N° 4 (04/2022)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVII, N° 4 (04/2022) . - p. 153-163 Titre : Antibacterial properties of several lichen extracts against two moderately halophilic bacteria from salted sheepskins Type de document : texte imprimé Auteurs : Ipek Türkmenoglu, Auteur ; O. Toksöz, Auteur ; Didem Berber, Auteur ; Pinar Caglayan, Auteur ; Meral Birbir, Auteur ; Nüzhet Cenk Sesal, Auteur Année de publication : 2022 Article en page(s) : p. 153-163 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Antibactériens Bactéries halophiles Cuirs et peaux de moutons Essais (technologie) Extraction (chimie) Extraits de plantes Lichens et constituants Statistique Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : It is well known that possible undesirable defects in finished leathers can result from microbial activities on the salted raw hides/skins during storage. The traditionally used salt curing method can control bacterial activities on the raw stock, but it does not seem possible to completely eliminate microbial attacks. Moderately halophilic bacteria can cause serious damage to hides/skins. From this point of view, potential procedures such as applying new antibacterial agents in the leather industry should be considered. Since some lichen species have been indicated for their promising biological efficacies in the literature; most researchers have focused on their potencies in various fields including leather industry from ancient times. In this study, the bacterial growth of proteolytic and lipolytic Gram-negative moderately halophilic bacteria, Chromohalobacter canadensis (YN6) and Halomonas eurihalina (BL5), from salted sheepskin samples were tested with the extracts of Usnea sp., Plastismatia glauca, Ramalina farinacea, Evernia divaricata, Bryoria capillaris, Hypogymnia tubulosa, Pseudevernia furfuracea and Lobaria pulmonaria. Some of these lichen species were found to be successful to inhibit the bacterial growth of C. canadensis (YN6) and H. eurihalina (BL5). In conclusion, lichen extracts may be utilized in stacked raw hides/skins in tanneries or warehouses to control moderately halophilic bacteria which causes several defects on leather. Note de contenu : - Moderately halophilic test bacteria - Lichen samples - Extraction of lichen samples - Antibacterial tests - Statistical analysis DOI : https://doi.org/10.34314/jalca.v117i4.4898 En ligne : https://drive.google.com/file/d/1JpK270mPD-iuFdcBl9PUzESigAqLbYrc/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37562 [article]

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Characterization and control of antibiotic-resistant calmati saltern's isolates with bacteriocins / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVII, N° 8 (08/2022)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVII, N° 8 (08/2022) . - p. 315-329 Titre : Characterization and control of antibiotic-resistant calmati saltern's isolates with bacteriocins Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur Année de publication : 2022 Article en page(s) : p. 315-329 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification Bactéries -- Isolement Bactéries halophiles Bactériocines Les bactériocines sont une famille de peptides ou protéines synthétisés naturellement par certaines bactéries. Une bactériocine consiste généralement en un composé protéique de 20 à 60 acides aminés. Les bactériocines ne sont pas des antibiotiques mais elles possèdent des propriétés antibiotiques : - Elles peuvent être bactériocides, c'est-à-dire éliminer certains micro-organismes. - Elles peuvent être bactériostatiques, c'est-à-dire inhiber la croissance de certains micro-organismes. Caractérisation Concentration bactéricide minimale La concentration bactéricide minimale (CBM) est la plus faible concentration d'un agent antibactérien nécessaire pour tuer une bactérie particulière1. Elle peut être déterminée à partir des tests de concentration minimale inhibitrice (CMI) de dilution en bouillon en repiquant les bactéries sur des plaques d'agar qui ne contiennent pas l'agent antibactérien que l'on teste. La CBM est identifiée en déterminant la concentration la plus faible d'agent antibactérien qui réduit la viabilité de l'inoculum bactérien initial à un niveau supérieur ou égal à 99,9%. La CBM est complémentaire à la CMI ; alors que le test CMI indique le niveau le plus bas d'agent antimicrobien qui inhibe la croissance, le CBM indique le niveau le plus bas d'agent antimicrobien qui entraîne la mort microbienne. Cela signifie que même si une CMI particulière montre une inhibition, le repiquage des bactéries sur de la gélose peut encore entraîner la prolifération de l'organisme car l'antimicrobien n'a pas causé la mort. Les agents antibactériens sont généralement considérés comme bactéricides si la CBM ne dépasse pas quatre fois la CMI. Comme le test CBM utilise des unités formant colonies comme mesure indirecte de la viabilité bactérienne, il peut être biaisé par des agents antibactériens qui provoquent l'agrégation des cellules bactériennes. On peut citer en exemples les flavonoïdes et les peptides. (Wikipedia) Concentration minimale inhibitrice (antimicrobiens) En microbiologie, la concentration minimale inhibitrice (CMI) est la plus faible concentration d'un produit chimique, généralement un médicament, qui empêche la croissance visible d'une ou de plusieurs bactéries. La CMI dépend du micro-organisme considéré, de l'être humain affecté (in vivo uniquement) et de l'antibiotique lui-même. La CMI est déterminée en préparant des solutions du produit chimique in vitro à diverses concentrations croissantes, en incubant les solutions avec des groupes séparés de bactéries en culture et en mesurant les résultats en utilisant une méthode de dilution standardisée (agar ou microdilution). Les résultats se classent ensuite comme "sensible", "intermédiaire" ou "résistant" à un antimicrobien particulier en utilisant un point d'arrêt. Les points d'arrêt sont des valeurs convenues, publiées dans les directives d'un organisme de référence, comme le US Clinical and Laboratory Standards Institute (CLSI), la British Society for Antimicrobial Chemotherapy (BSAC) ou le Comité européen sur les tests de sensibilité aux antimicrobiens (EUCAST). On a pu constater des écarts importants au niveau des points d'arrêt de divers pays européens au fil des ans, et entre ceux de l'EUCAST et du CLSI. Alors que la CMI est la concentration la plus faible d'un agent antibactérien nécessaire pour inhiber la croissance visible, la concentration bactéricide minimale (CBM) est la concentration minimale d'un agent antibactérien qui entraîne la mort bactérienne. Plus la CMI est proche de la CBM, plus le composé est bactéricide. La première étape de la découverte d'un médicament est souvent le dépistage d'un médicament candidat de banque de données pour les CMI contre les bactéries d'intérêt. En tant que tels, les CMI sont généralement le point de départ pour de plus grandes évaluations précliniques de nouveaux agents antimicrobiens. Le but de la mesure de la concentration minimale inhibitrice est de s'assurer que les antibiotiques sont choisis efficacement pour augmenter le succès du traitement. (Wikipedia) Microbiologie Résistances aux antibiotiques Sels de sodium Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Camalti Saltern is the largest solar saltern in Izmir, Turkey. The salt obtained from Camalti Saltern is used in food and leather industries to prevent bacterial growth. In this saltern, seawater is pumped into shallow ponds. Then, the sun and wind cause evaporation and crystallization, finally sea salt is produced. Due to the fact that Camalti Saltern contains various halophilic bacteria, the goals of the present study were to isolate and identify haloversatile bacteria from Camalti Saltern’s brine samples, to examine their antibiotic resistance profiles, to determine antimicrobial activities under optimum environmental conditions, to determine bacteriocin concentration by Bradford Method, to detect Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) of bacteriocins against multidrug-resistant isolates, and to observe the cell structure of bacteriocin-treated bacteria under SEM. Sixteen bacterial isolates were recovered from Camalti Saltern’s brine samples and were identified as 14 different species (Bacillus haynesii, Bacillus simplex, Bacillus subtilis subsp. stercoris, Bacillus pumilus,Staphylococcus petrasii subsp. jettensis, Staphylococcus saprophyticus subsp. saprophyticus, Kocuria sediminis, Rhodococcus enclensis, Marinobacter hydrocarbonoclasticus, Vibrio olivae, Marinomonas communis, Pseudomonas psychrotolerans, Salinivibrio costicola subsp. vallismortis, Vibrio neocaledonicus). Percentages of antibiotic resistance of isolates were 63% to aztreonam, 50% to amoxicillin/clavulanic acid, 44% to ampicillin, 44% to cefadroxil, 31% to imipenem, 19% to ampicillin/sulbactam, 6% to chloramphenicol, 6% to tetracycline, 6% to mupirocin, 6% to meropenem. The bacteriocin concentrations of Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis were measured as 1.02 mg/mL and 1.25 mg/mL, respectively. Bacteriocins of Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis, which were not resistant to any antibiotics tested,exhibited the inhibitory effect against Kocuria sediminis resistant to ten antibiotics and Bacillus pumilus resistant to four antibiotics. Bacteriocin of Salinivibrio costicola subsp. vallismortis also demonstrated the inhibitory effect against Pseudomonas psychrotolerans resistant to five antibiotics. Scanning electron micrographs showed that cell morphologies of bacteriocin-treated isolates (Kocuria sediminis, Bacillus pumilus, Pseudomonas psychrotolerans) were damaged. In conclusion, bacteriocins produced from the haloversatile Camalti saltern isolates may be used in the leather industry to prevent the growth of antibiotic-resistant haloversatile bacteria. Note de contenu : - Sample collection - Isolation of bacteria - Characterization of the isolates - Amplification and sequencing of 16S rRNA genes - Nucleotide accession number - Antibiotic susceptibility - Preparation of cell-free supernatant - Screening of antimicrobial activity among the test isolates - Determination of protein concentration using Bradford method - Optimum conditions for bacteriocin production from Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis - Effect of heat on bacteriocins produced by Rhodococcus enclensis and Salnivibrio costicola subsp. vallismortis - Effect of pH on bacteriocins produced by Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis - Effect of NaCl concentration on bacteriocins produced by Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis - Effect of enzymes (Proteinase K and Lipase) on bacteriocins produced by Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis - Minimum inhibitory concentrations and minimum bactericidal concentrations of bacteriocins against multidrug-resistant isolates - Preparation of bacteriocin-treated and bacteriocin-untreated cells for scanning electron microscopy - Table 1 : Characteristics of haloversatile bacteria isolated from brine samples of the Camalti Saltern - Table 2 : Inhibition zone diameter measurements (mm) of haloversatile bacteria isolated from brine samples of the Camalti Saltern - Table 3 : Inhibitory effect of bacteriocin produced by bacterial isolates against each other - Table 4 : Inhibition zone diameters (mm) of multidrug-resistant haloversatile bacteria against bacteriocins obtained from Rhodococcus enclensis, Salinivibrio Costicola subsp. vallismortis at different incubation temperatures, pH and NaC1 concentrations - Table 5 : The effects of different temperatures, pH, NaC1 concentrations, proteinase K and lipase enzymes on antibacterial effect of bacteriocins - Table 6 : The Minimum Inhibitory Concentrations of bacteriocin produced from Rhodococcus enclensis against Kocuria sediminis and Bacillus pumilus - Table 7 : The Minimum Inhibitory Concentrations of bacteriocin produced from Salinivibrio costicola subsp. vallismortis against Kocuria sediminis, Bacillus pumilus and Pseudomonas psychrotolerans DOI : https://doi.org/10.34314/jalca.v117i8.5982 En ligne : https://drive.google.com/file/d/1G3HZoIp4GH7W3A22JxMjjbmRimXH0dkk/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37824 [article]

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Controlling the growth of extremely halophilic bacteria on brine cured cattle-hides / Meral Birbir in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 84, N° 5 (09-10/2000)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 84, N° 5 (09-10/2000) . - p. 201-204 Titre : Controlling the growth of extremely halophilic bacteria on brine cured cattle-hides Type de document : texte imprimé Auteurs : Meral Birbir, Auteur ; D. G. Belay, Auteur Année de publication : 2000 Article en page(s) : p. 201-204 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Antibactériens Bactéries -- Croissance Bactéries halophiles Cuirs et peaux -- Conservation Cuirs et peaux de bovins Tags : Naphtalène  Dichlorobenzène  'Trichloro-S-triazinetrione'  'sulfate  de  sodium'  'Bisulfite  sacide  acétique  'Ethoxylate  diméthyle  potassium  dithiocarbamate  '2(thiocyanométhylthio)benzothiazole'  'Bis(thiocyanate)  méthylène'  'Ortho-phénylphénate  'Ortho-benzyl-para-chlorophénol' Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Six commercially available brine raceway bactericides were tested for their ability to prevent the growth of extremely halophilic bacteria isolated from hides that were brine cured in either the US or Canada. These products were used at their recommended raceway doses and at two times and four times that amount. The active ingredients of the commercially available bactericides were: naphthalene and 1-2 dichlorobenzene (Bactericide A), trichloro-S-triazinetrione and sodium sulphate (Bactericide B), sodium bisulfite and acetic acid (Bactericide C), alkyl phenol ethoxylate and potassium dimethyl dithrocarbonate (Bactericide D), 2(thiocyanomethylthio) benzothiazole and methylene bis (thiocyanate) (Bactericide E), sodium-ortho-phenylphenate and ortho- benzyl-para-chlorophenol (Bactericide F). Bactericides A, B and E were found to be effective in preventing growth of the extremely halophilic bacteria tested. Bactericides C, D and F were found to be ineffective at the concentrations tested. All of the extremely halophilic isolates used in this experiments showed positive gelatinase activity. Note de contenu : - Table 1 : Bactericides tested on extremely halophilic bacteria - Table 2 : Effect of bactericides on extremely halophilic bacterial growth En ligne : https://drive.google.com/file/d/1l9lBTBXX8_j6sZDgznrd2XZEqkhforCw/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40917 [article]

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Examination of moderately halophilic bacteria / Meral Birbir in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 105, N° 5 (09-10/2021)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 105, N° 5 (09-10/2021) . - p. 231-241 Titre : Examination of moderately halophilic bacteria Type de document : texte imprimé Auteurs : Meral Birbir, Auteur ; Kubra Kizilkaya, Auteur ; Pinar Caglayan, Auteur Année de publication : 2021 Article en page(s) : p. 231-241 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bactéries -- Comptage Bactéries halophiles Cendres Cuirs et peaux -- Conservation Cuirs et peaux -- Microbiologie Humidité Peaux brutes -- Salage pH Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The salt curing process may cause contamination of skin with micro-organisms found in the salt. Moderately halophilic bacteria are among the dominant halophilic micro-organisms which adversely affect skin quality. Therefore, the study was focused to examine prevalence and total numbers of aerobic bacteria, moderately halophilic bacteria, proteolytic and lipolytic moderately halophilic bacteria in curing salt samples and examine coiony characteristics and biochemical reactions of moderately halophilic bacteria. Twenty-five salt sampies that are used in the preservation of hides and skins were collected from different leather factories in Corlu (20 salt sampies) and Tuzla (5 salt samples) Leather Organized Industrial Zones in Turkey. The pH, ash and moisture values of the salt sampies were respectively determined as between 6.60-7.95, 90%-96%, 2.1%-2.9%. The total numbers of aerobic bacteria, moderately halophilic bacteria, proteolytic and lipolytic moderately halophilic bacteria in the sampies were detected as 2-70 CFU/g, 1-13 CFU/g, 1-13 CFU/g and 1-4 CFU/g, respectively. Ninety-five moderately halophilic bacterial isolates were recovered from the salt sampies. Three, thirteen, seventeen, fifty-six, two, four, five percent of the bacterial colonies were dark yellow, pink, white, cream, beige, dark cream and yellow, respectively. Seventy-nine, sixteen, four and one percent of the bacterial colonies were found as circuiar, punctiform, irregular and rhizoid, respectively. Sixty-six, thirty-four, forty-five and fifty-five percent of the isolates were Gram-positive, Gram-negative, cocci-shaped, and rod-shaped cells. Fifty-four and thirty-eight percent of the isolates were motile and endospore-forming bacteria, respectively. Ail isolates grew on complex agar media containing 3-15% NaCI (w/v) concentration but did not grow on complex agar medium without NaCI and complex agar medium with 30% NaCI. White optimal growth of eighty-two percent of the isolates were observed at Complex agar medium with 10% NaCI, eighteen percent of isolates exhibited optimal growth at Complex agar medium with 10-15% NaCI. All isolates were catalase-positive. Sixty-one, seventy-four, twenty-five, sixty percent of the isolates produced oxidase, protease, lipase, and amylase enzymes, respectively. Thirty-three of percent of the isolates produced ammonia from peptone. ln conclusion, ail salt sampies contained moderately halophilic bacteria, 92% and 48% of salt samples comprised respectively proteolytic and lipolytic moderately halophilic bacteria. Therefore, destructive moderately halophilic bacteria in each salt should be removed using antimicrobial applications before curing process to prevent damage to the salted hides and skins during storage. Note de contenu : - Salt samples - Determination of pH values, moisture and ash contents of salt samples - Determination of numbers of aerobic bacteria, moderately halohilic bacteria, proteolytic moderately halophilic bacteria and lipolytic moderately halophilic bacteria in salt samples - Biochemical ativities of moderately halophilic bacterial isolates - Table 1 : The pH values, ash contents, moisture content, total counts of aerobic bacteria and moderately halophilic bacteria of the salt samples - Table 2 : Bacterial colony characteristics of pure moderately halophilic bacterial isolates - Table 3 : Biochemical test results of moderately halophilic bacterial isolates En ligne : https://drive.google.com/file/d/1fiuvgEaAYneIoA1jwQhL4a7iKewvC3nP/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=36349 [article]

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Extracellular protease activities of extremely halophilic archaea and their control via direct electric current / Meral Birbir in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 92, N° 2 (03-04/2008)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 92, N° 2 (03-04/2008) . - p. 53-58 Titre : Extracellular protease activities of extremely halophilic archaea and their control via direct electric current Type de document : texte imprimé Auteurs : Meral Birbir, Auteur ; Z. B. Ozdogru, Auteur ; Yasar Birbir, Auteur ; Ayse Ogan, Auteur Année de publication : 2008 Article en page(s) : p. 53-58 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bactéries halophiles Bain de saumure Courants continus Cuirs et peaux -- Conservation Enzymes protéolytiques Une enzyme protéolytique est une enzyme capable de couper une protéine en plusieurs fragments ou peptides. La trypsine, la papaïne, la pepsine, la chymotrypsine, la plasmine, la subtilisine... sont capables de couper une protéine, chaque enzyme étant spécifique de certains sites particuliers de cette protéine. C'est ainsi, par exemple, qu'une immunoglobuline G est découpée par la papaïne en un fragment Fc et deux fragments Fab, comme l'a montré Porter en 1959. Microbiologie Peptidases Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Proteolytic enzymes which are produced by extremely halophilic Archaea present in salt may cause serious damage on salted hides and may result in significant economic losses in the leather industry. Hence, protease activities of 24 protease producing extremely halophilic archaeal strains isolated from Tuz Lake, Kaldırım and Kayacık salterns and Tuzköy salt mine were determined in this study. The protease activities of Tuz Lake, Kaldırım saltern, Kayacık saltern and Tuzköy salt mine strains were found as between 80.0 and 83.3, 81.1 and 83.8, 83.3 and 83.4, 82.0 and 83.4 Units, respectively. To prevent proteolytic haloarchaeal damage on brine cured hides, the protease producing strains were treated with 0.5A direct electric current for 20min. Although proteolytic strains of Tuz Lake and Tuzköy salt mine in the liquid gelatin media were killed by 0.5A direct electric current within 15min, 10min direct electric current treatment were enough to kill all proteolytic strains of Kaldırım and Kayacık salterns. Also, the effect of 0.5A direct electric current on protease activities of the test strains was examined during 20min treatment in this study. It was found that 0.5A direct electric current caused a slight decrease on protease activities of the test strains. Therefore, it is necessary to kill protease producing strains in brine solution with direct electric current before the excretion of their protease into the brine solution. Note de contenu : - Extremely halophilic strain - Protease activities of the extremely halophilic strains - Inactivation of the test strains with 0.5A direct electric current - Table 1 : Protease activities of extremely halophilic Archaea isolated from Tuz Lake, Kaldırım and Kayacık Salterns and Tuzköy Salt Mine - Table 2 : Voltage, temperature and rf values of 4 mixed proteolytic halophilic Archaea inactivated in the liquid gelatin media by 0.5A direct electric current within 20 minutes - Table 3 : The effect of 0.5A direct electric current on protease activities of some of the test strains during 20 minutes treatment En ligne : https://drive.google.com/file/d/1gBbMoBdjmpLA6cnCaGdQjxhmAhOnxKZz/view?usp=share [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=38930 [article]

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Identification of slightly halophilic bacteria from salted sheepskin samples and investigation of their biotechnological importance / Dilek Yalcin in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 108, N° 1 (01-02/2024)  

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Inactivation of moderately halophilic soak liquor / Pinar Yilmaz in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 108, N° 4 (07-08/2024)  

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Isolation and identification of moderately halophilic bacteria from soak liquor samples collected of leather tanneries / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVIII, N° 7 (07/2023)  

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Prevention of halophilic microbial damage on brine cured hide by extremely halophilic halocin producer strains / Meral Birbir in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 88, N° 3 (05-06/2004)  

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