| Titre : |
Identification and characterization of potential biocide-resistant fungal strains from infested leathers - A systematic study |
| Type de document : |
texte imprimé |
| Auteurs : |
Kavitha Sundar, Auteur ; Yasmin Khambhaty, Auteur ; Chandra Babu Narasimhan Kannan, Auteur |
| Année de publication : |
2020 |
| Article en page(s) : |
p. 39-48 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Aspergillus
Caractérisation
Champignons microscopiques
Cuirs et peaux -- Conservation
Résistance aux antimicrobiens
Wet-blue (tannage)Peau tannée au chrome (le chrome donne une couleur bleue)
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
This study is aimed at identification of biocide tolerant/resistant fungal strains afflicting the leather industry. Fungal infestation occurs sometimes despite biocide treatment during leather processing. This persistent growth can be due to the development of biocide resistance which can lead to health hazards and economic loss. As no study has so far been reported to either confirm this or to identify such fungal strains, a systematic approach has been made in this study to address these aspects. Fungal strains were collected from infested leathers from tanneries to identify biocide resistant fungal strains afflicting leather industry. Phenotypic characterization revealed Aspergillus as the most dominant with 58% occurrence. Ten isolates were subjected to 18s rRNA sequencing and four strains were identified as Aspergillus niger. An in-vitro susceptibility to four leather fungicides was assessed to identify the biocide tolerant strains. S-6 A. niger strain was found to be the most tolerant as evidenced by high MIC (7.81µg ml-1) against the most effective biocide, 2-(thiocyanomethylthio) benzothiazole. In-vivo studies on chrome-tanned leathers also confirmed this finding. SEM studies revealed considerable morphological changes in S-6 compared to wild strain providing further evidence that it may have developed biocide resistance. |
| Note de contenu : |
- MATERIALS AND METHODS : Source of media and chemicals - Collection of fungal infected leather samples from tanneries - Isolation and maintenance of fungal cultures - Phenotypic characterization - Genotypic identification and phylogenetic analysis - In vitro susceptility of the fungal strans against selected biocides - Studies on the growth of resistant strains on biocide treated leather - Scanning electron microscopy (SEM) analysis
- RESULTS AND DISCUSSION : Phenotypic characterization - Genotypic characterization - In vitro susceptibility of the fungal strains against selected biocides - SEM analysis - Evaluation of growth of A. niger strains on biocide treated wet-blue leathers |
| DOI : |
https://doi.org/10.34314/jalca.v115i2.1483 |
| En ligne : |
https://drive.google.com/file/d/1OlDyUj9jtT8B0doew2qNKyYGrKhBm5KY/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33889 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXV, N° 2 (02/2020) . - p. 39-48
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