[article]
| Titre : |
Scanning probe microscopy. A useful tool in leather research |
| Type de document : |
texte imprimé |
| Auteurs : |
G. Reich, Auteur ; T. Taeger, Auteur ; W. Pompe, Auteur ; M. Mertig, Auteur ; J. Bradt, Auteur |
| Année de publication : |
1998 |
| Article en page(s) : |
p. 11-14 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Optical microscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) are widely accepted investigation methods in fundamental and applied leather research. They are under further development, for instance by cryotechnique (cryomicroscopic SEM), rotary shadowing-TEM, use of confocal laser sanner and computer aided microscopy in general. The further possibilities and the limits of this method are physically substantiated and illustrated.
Currently, novel methods of scanning probe microscopy have attracted special attention in physics, chemistry, material science and biology, because they can resolve surface details down to atomic level. Especially, scanning for microscopy (SFM) shows the capability to image isolating surfaces, including biological specimens and coatings, with nanometer resolution. The main advantages for applications in biology are : the possibility of working in a liquid environment under physiological conditions, to get three-dimensional topographic information and the possibility of probing local forces e.g. to obtain information about the mechanical properties of the investigated specimen.
We have carried out SFM of leather. The investigations started with collagen monomers and collagen fibrils immobilized as different substrates. So far the best resolution we could achieve is to image rod-like individual collagen monomers with a diameter of 1.6 nm and the 67 nm-periodicity of native type I collagen fibrils. Several fibrils are twisted into bundles in the form of a right-handed matrix. Changes in fibril morphology after calcification have been investigated. The results show that mineralisation occurs with higher density mainly at crossover regions of collagen fibrils. It has been found that the formed hydroxyapatite crystallites are similar in size, typically between 50 and 100 nm.
Investigation on leather samples after various treatments demonstrate that the applied method is also suited to investigate more complex samples. The advantage of SFM in comparison to SEM is the possibility of use to investigate wet samples and to obtain topographic information with high imaging value.
In conclusion scanning probe microscopy, especially the scanning force microscopy (SFM), is recommended as an useful tool in leather research in addition to the traditional microscopic investigation methods. |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=7936 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 82, N° 1 (01-02/1998) . - p. 11-14
[article]
|
Aller sur edunet
Accueil
Ajouter le résultat dans votre panier Affiner la rechercheScanning probe microscopy. A useful tool in leather research / G. Reich in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 82, N° 1 (01-02/1998)
