Measurement of the proteolytic activity of bate in drum liquor / Samuel M. Mozersky in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. LXXXVIII, N° 8 (08/1993)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. LXXXVIII, N° 8 (08/1993) . - p. 283-290 Titre : Measurement of the proteolytic activity of bate in drum liquor Type de document : texte imprimé Auteurs : Samuel M. Mozersky, Auteur ; David G. Bailey ; Marvin A. Baynes, Auteur Année de publication : 1993 Article en page(s) : p. 283-290 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675.2 Préparation du cuir naturel. Tannage Résumé : A colorimetric procedure has been developed for assay of the proteolytic activity of bate in drum liquor using hide powder azure, a commercially available substrate. Stock bate powders or solutions can be assayed with the same procedure.Results of the application of the assay to tannery samples are presented and the potential use of the procedure for quality control is discussed. The cost of chemicals and single-use equipment for a single assay is $4.00, and the run time 30 minutes.Fifteen samples can be run by one operator "simultaneously", i.e., with a one minute delay between one sample and the next. The procedure has a relative standard deviation of less than 5%. En ligne : https://drive.google.com/file/d/1miUnWJSGsqYYVCt5WYXxhmeDQAO4DSYX/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=8161 [article]

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Optimization of the assay of bate and drum liquor for proteolytic activity / Samuel M. Mozersky in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. LXXXX, N° 8 (08/1995)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. LXXXX, N° 8 (08/1995) . - p. 235-242 Titre : Optimization of the assay of bate and drum liquor for proteolytic activity Type de document : texte imprimé Auteurs : Samuel M. Mozersky, Auteur ; Marvin A. Baynes, Auteur ; David G. Bailey, Auteur Année de publication : 1995 Article en page(s) : p. 235-242 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : In two recent publications from this laboratory, procedures were described for measuring the proteolytic activity of bate and (bate-containing) drum liquor using hide powder azure (HPA) as substrate. Subsequent to that work, it was found that HPA sometimes loses much of its sensitivity to proteolytic enzymes of storage. This led to a study of the substrate and of related factors affecting the assay, which resulted in substantial revision of the assay procedure, as follows. HPA is activated prior to use by reduction of particle size and a mild heat treatment, and200mg used per assay (instead of 50mg). A clarified extract of the sample to be assayed is introduced into the incubation tube (a 25 mm ´ 150 mm screw-cap tube) instead of the crude material. Since proteolytic activity was found to change by 10 to 15% per °C, good temperature control is necessary; for research purposes, a water-bath shaker-incubator operating at 37°C and 400 rpm is recommended. Incubation time has been reduced from 20 min to 10 min at ambient temperature (ca. 25°C), 5 min at 37°C. These modifications have resulted in the following advantages: (1) substantially increased sensitivity, (2) a linear dependence of the color measured on enzyme concentration, and (3) improved reproducibility. En ligne : https://drive.google.com/file/d/1m4ZmYp-cbBTCpJwnoL3XdK7s8DMhbC5P/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=7931 [article]

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