[article]
| Titre : |
Alpha-glucosylrutin (AGR) - An innovative antioxidant in skin protection |
| Type de document : |
texte imprimé |
| Auteurs : |
F. Stäb, Auteur ; R. Wolber, Auteur ; C. Mundt, Auteur ; T. Blatt, Auteur ; K. Will, Auteur ; R. Keyhani, Auteur ; F. Rippke, Auteur ; H. Max, Auteur ; U. Schönrock, Auteur ; H. Wenck, Auteur ; I. Moll, Auteur ; E. Hölzle, Auteur ; K. P. Wittern, Auteur |
| Année de publication : |
2000 |
| Article en page(s) : |
p. 39-43 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
For strategies of active skin protection against UV-light and reactive oxygen species (ROS), the topical application of antioxidants becomes more and more prominent (5,11). However, only some of the in vitro effective antioxidants, appear to be functional in vivo too (12). In our studies we compared physiological activities of diverse known antioxidants (e. g. Vit. E, C) versus the highly water soluble flavonoid alpha glucosylrutin (AGR). The studies were conducted in vivo on human skin and in vitro using primary cells from biopsies of human skin. In vitro results: 1) AGR can bolster and protect the intracellular thiol level and the mitochondrial membrane potential of primary keratinocytes and fibroblasts against depletion by oxidative stress. 2) AGR deminishes the oxidative stress-induced decline in the rate of DNA-synthesis, -protection and -repair. 3) AGR reduces the level of tyrosine phosphorylation and ERK1/2 activation in primary skin cells challenged by oxidative stressors (H2O2, UVA). 4) AGR protects primary human skin cells against both, UV-induced reduction in DNA- and hyaluronan- synthesis as well as against UV-induced intracellular hydroperoxide formation. In vivo results: 1) Our studies on normal human skin using UVA-induced ultraweak photon emission measurements (UPE) confirm the antioxidative potential of topical AGR in vivo. 2) After UVA-irradiation in vivo, the phosphorylation of intracellular tyrosine residues and the hydroperoxide levels are significantly enhanced in keratinocytes isolated from donors suffering from polymorphic light eruption (PLE) compared with keratinocytes from healthy donors, but can be reduced even in PLE-skin cells by topical pretreatment with AGR-preparations. 3) Comparative in vivo studies on equimolar ratios of AGR versus Parsol 1789 using reflexion spectrometry give evidence that topical AGR acts predominantly as an antioxidant and not as an UV-filter. 4) Several clinical trials and home in use tests on PLE prophylaxis and UVA-protection of healthy skin showed a significant improvement of the PLE and the UVA-erythema scores at skin sites pretreated with AGR compared with untreated or placebo treated skin sites. |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10799 |
in IFSCC MAGAZINE > Vol. 3, N° 4 (10-11-12/2000) . - p. 39-43
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Alpha-glucosylrutin (AGR) - An innovative antioxidant in skin protection in IFSCC MAGAZINE, Vol. 3, N° 4 (10-11-12/2000)
