| Titre : |
In-vivo measurements of free radicals in human skin |
| Type de document : |
texte imprimé |
| Auteurs : |
Leonhard Zastrow, Auteur ; Thomas Herrling, Auteur ; Lawrence J. Berliner, Auteur ; Louis Ferrero, Auteur ; Norbert Groth, Auteur |
| Année de publication : |
2003 |
| Article en page(s) : |
p. 295-301 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Tags : |
'Radicaux libres' Résonance spin électronique' 'Nitroxide étiquette par rotation' 'Irradiation UVA-UVB' |
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
UVA-UVB generated free radicals in human skin were measured by Electron Spin Resonance (ESR) spectroscopy and imaging. The short lifetime of the generated free radicals and reactive oxygen species like •OH−; O2-• and L• demands the application of spin traps (nitroxide spin labels) for scavenging and accumulating to get a sufficient Signal-to-Noise ratio. The ESR spectroscopy and imaging measurements showed that the free radicals in skin were mainly generated in the dermal layer by UVA. Using ESR measurements, the protective effect of different UVB-UVA filter combinations and antioxidants in the skin could be clearly assessed. The used microwave frequencies of 9.5 GHz (λ=350-600 nm) and 3 GHz (λ=9.5 nm) enabled an undisturbed investigation of the whole skin up to the lower dermis in contrast to optical detection methods that are more limited in depth (λ=350-600 nm). ESR spectroscopy is a valuable method for the evaluation of the UV-protection capacity of sunscreen products. |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10552 |
in IFSCC MAGAZINE > Vol. 6, N° 4 (10-11-12/2003) . - p. 295-301
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In-vivo measurements of free radicals in human skin in IFSCC MAGAZINE, Vol. 6, N° 4 (10-11-12/2003)
