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Identification of decorin and other proteins in bovine hide during its processing into leather / Mila L. Adelma-Ramos in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CIII, N° 10 (10/2008)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CIII, N° 10 (10/2008) . - p. 324-329 Titre : Identification of decorin and other proteins in bovine hide during its processing into leather Type de document : texte imprimé Auteurs : Mila L. Adelma-Ramos, Auteur ; Laurie L. Fortis, Auteur ; Renée J. Latona, Auteur Année de publication : 2008 Article en page(s) : p. 324-329 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The present work attempts to develop a more accurate and reliable assay for decorin concentration in hides as they are processed to leather. The ELISA technique previously developed for decorin analysis is further improved and optimized by treating the samples with chondroitinase-ABC after dialyzing therm in the presence of collagenase. Come newer techniques developed and adapted include SDS-PAGE and western blottingusing immuno-colorimetric staining. Probing the blotted protein with a combination of two different antibodies specific for decorin, i.e. PK1 and 6D6, is more specific and sensitive than using either antibody individually. Western blotting can be used visually and quantitatively to confirm that ELISA-detected species are indeed decorin. The intact proteoglycan as wall as the core protein and resulting fragmentations of decorin are determined after treating the samples with collagenase and chondroitinase ABC alone or a combination of these two enzymes. The fate of the different proteins in raw hides to leather is followed by analyzing the separated protein bands in SDS-PAGE gel using MALTI-TOF peptide mapping. En ligne : https://drive.google.com/file/d/1s5W3-EUAHSTOf1eqDmkzW4x0oQtJYeg6/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=2408 [article]

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Ultrasound assisted diffusion in vegetable tanning for leather processing / Venkatasubramanian Sivakumar in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CIII, N° 10 (10/2008)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CIII, N° 10 (10/2008) . - p. 330-337 Titre : Ultrasound assisted diffusion in vegetable tanning for leather processing Type de document : texte imprimé Auteurs : Venkatasubramanian Sivakumar, Auteur ; M. V. Harikrishnan, Auteur ; K. Gopi, Auteur Année de publication : 2008 Article en page(s) : p. 330-337 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675.2 Préparation du cuir naturel. Tannage Résumé : Vegetable tanning is one of the more versatile tanning methodologies involving natural materials. The problem associated with this tanning method is that it takes longer time for diffusion of vegetable tanning agents (VTA) thoug the pelt matrix. Therefore, there is a pressing need to transform this traditional eco-friendly tanning process into a tanner friendly efficient methodology by way of augmentation techniques such as the use of ultrasound. In the present paper, influence of ultrasound on vegetable tanning process has been studied. The influence of important process parameters such as process time, ultrasonic power, pre-tanning on VTA uptake has been investigated. The effect of ultrasonic power on shrinkage temperature of tanned leather has also been studied. The results indicate that there is a significant enchancement in vegetable tanning process due to the use of ultrasound. There is about 30-40 % increase in % uptake of VTA of VTA due to the use of ultrasound for 120-210 watts. In order to find out the possible mechanism for the enhancement with ultrasound, effect of pre-sonication of substrate (pelt matrix) as well as substance (VTA) has been studied. There is an appreciable reduction in the particle-size of VTA due to sonicationprior to tanning process aiding penetration. diffusion coefficient (D) of VTA during the tanning process has been calculated from the experimental data which indicates nearly two fold increase in the D value 2.64 x 10-5 cm2/sec) due to ultrasound under the given process conditions. Therefore, ultrasound application in vegetable tanning proccess could improve the diffusion rate leading to significant savings in process time and enhancementn in quality of the tanned leather. This methodology can be easily adopted in existing paddle or tanning pit systems for large scale production. Hence, this novel technique is a viable option for tanners in near future. En ligne : https://drive.google.com/file/d/1oPyAT4I2Qw5rbeLgjuo6crxAzjyIEY41/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=2409 [article]

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Enzymatic dehairing of cattlehide with an alkaline protease isolated from aspergillus tamarii a,b / Daynandan Anandan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CIII, N° 10 (10/2008)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CIII, N° 10 (10/2008) . - p. 338-344 Titre : Enzymatic dehairing of cattlehide with an alkaline protease isolated from aspergillus tamarii a,b Type de document : texte imprimé Auteurs : Daynandan Anandan, Auteur ; William N. Marmer, Auteur ; Robert L. Dudley, Auteur Année de publication : 2008 Article en page(s) : p. 338-344 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : An enzymatic dehairing protocol based on the alkaline serine protease, siolated from the fungus aspergillus tamarii, required 16h, and we observed concomitant grain damage. The use of sodium dodecyl sulfate (SDS) as a preatreatment to remove the lipids from the hide allowed a shortening of the dehairing time to 6h without grain damage. We postulated that the SDS removed all of the sebaceous grease from the pores of the hair facilitating the penetration of the enzyme through the grain layer Using a lypophilic dye, nile red, we showed that SDS did remove some of the grease from the grain side of the hide. By tagging the enzyme with a fluorescent label, we clearly showed, however, that the enzyme penetrated the hide only through its flesh side. The enzymatic dehairing proccess did not remove the fine hairs frome the hide. Adding a common sharpening agent, sodium sulfide, to the liming step removed the fine hairs but also caused grain damage. Employing an auxiliary oxidative dehairing step removed the fine hairs but also caused grain damage. Employing an auxiliary oxidative dehairing step, based on alkaline sodium percarbonate, did remove the fine hairs ; under carefully controlled conditions, grain damage wa not observed. Mechanical data were collected from leather prepared from enzymatically dehaired hide that had been limed, enzymatically dehaired hide that had been further oxidatively dehaired and hide that had been dehaired with sulfide and relimed. The tensile strength of the leather made from enxymatically dehaired and oxidatively treated hide and the leather prepared from the enzymatically dehaired and traditionnaly relimed hide was not significantly different from the control. The leather made from the enzymatically dehaired and oxidatively treated hide, however, was significantly stronger than the leather prepared from the enzymatically dehaired and traditionally relimed hide. En ligne : https://drive.google.com/file/d/1dtUmmk8FB85gUuczxDAnfOackJJ9Nflp/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=2410 [article]

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Lightfastness properties of leathers tanned with various vegetable tannins / Hasan Ozgunay in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CIII, N° 10 (10/2008)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CIII, N° 10 (10/2008) . - p. 345-352 Titre : Lightfastness properties of leathers tanned with various vegetable tannins Type de document : texte imprimé Auteurs : Hasan Ozgunay, Auteur Année de publication : 2008 Article en page(s) : p. 345-352 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675.2 Préparation du cuir naturel. Tannage Résumé : The color changes of bovine leathers, tanned with commonly used hydrolysable and condensed vegetable tannins, were measured colorimetrically as a function of prolonged irradiation with xenon tet light. the results showed that hydrolysable and condensed tannins exhibited differences in their light fastnesses. The color change of hydrolysable tannins under the effect of light was considerably low when compared to the condensed tannins. Gambier and quebracho tannins, among the condensed tannins, exhibited a rapid color change in the direction of red and yellow,while mimosa showed a slower color shift. Tara, among the hydrolysable tannins, exhibited a slower color shift than the others. At the end of 24 hours, the color changes, observed as darkening in all the tannin groups reached a maximum point after which color lightening was observed with extended time. En ligne : https://drive.google.com/file/d/1VgXfgBdglQVC9trAOvAgGCSegcL990X1/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=2411 [article]

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